RGD Reference Report - Remodeling of experimental arteriovenous fistula with increased matrix metalloproteinase expression in rats. - Rat Genome Database

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Remodeling of experimental arteriovenous fistula with increased matrix metalloproteinase expression in rats.

Authors: Chan, CY  Chen, YS  Ma, MC  Chen, CF 
Citation: Chan CY, etal., J Vasc Surg. 2007 Apr;45(4):804-11.
RGD ID: 1642040
Pubmed: PMID:17398390   (View Abstract at PubMed)
DOI: DOI:10.1016/j.jvs.2006.12.063   (Journal Full-text)

OBJECTIVE: Venous dilatation and wall thickening are part of the maturation of an arteriovenous fistula (AVF). However, the underlying mechanism of AVF remodeling remains unknown. We therefore studied whether matrix remodeling elicited by matrix metalloproteinases (MMPs) may contribute to AVF maturation. METHODS: A femoral AVF model in rats was established by invagination of the distal end of the left femoral artery into the femoral vein after venotomy (fistula group). In the sham group, the left femoral artery was cut, but venous invagination was not performed. Changes in the hemodynamics and the diameter of the iliac vein were studied on days 3, 14, and 28, then the iliac vein was removed and examined for changes in wall thickness and expression of MMP-2 and MMP-9, type 4 tissue inhibitor of metalloproteinases (TIMP-4), and collagen I and III by immunohistochemical staining or Western blotting. RESULTS: Femoral AVF resulted in a sixfold increase in blood flow in the fistula iliac vein and a gradual, but significant, increase in the thickness of the intima and media and marked up-regulation of MMP-2 and MMP-9, down-regulation of TIMP-4, as well as degradation of collagens I and III. The collagen I/III ratio was significantly higher in the 14-day fistula group (1.44 +/- 0.32) than in the sham group (0.82 +/- 0.15) and was even higher in the 28-day fistula group (1.76 +/- 0.21). CONCLUSION: The present results confirmed our hypothesis that a high blood flow rate in the fistula vein affects the expression of MMPs and TIMP-4, resulting in the remodeling or maturation of the AVF. Remodeling is associated with degradation of collagen, with an increase in the collagen I/III ratio.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MMP2HumanArteriovenous Fistula  ISOMmp2 (Rattus norvegicus)protein:increased expression:ileal veinRGD 
MMP9HumanArteriovenous Fistula  ISOMmp9 (Rattus norvegicus) RGD 
Mmp2RatArteriovenous Fistula  IEP protein:increased expression:ileal veinRGD 
Mmp2MouseArteriovenous Fistula  ISOMmp2 (Rattus norvegicus)protein:increased expression:ileal veinRGD 
Mmp9RatArteriovenous Fistula  IEP  RGD 
Mmp9MouseArteriovenous Fistula  ISOMmp9 (Rattus norvegicus) RGD 
TIMP4HumanArteriovenous Fistula  ISOTimp4 (Rattus norvegicus)protein:decreased expression:ileal veinRGD 
Timp4RatArteriovenous Fistula  IEP protein:decreased expression:ileal veinRGD 
Timp4MouseArteriovenous Fistula  ISOTimp4 (Rattus norvegicus)protein:decreased expression:ileal veinRGD 

Objects Annotated

Genes (Rattus norvegicus)
Mmp2  (matrix metallopeptidase 2)
Mmp9  (matrix metallopeptidase 9)
Timp4  (TIMP metallopeptidase inhibitor 4)

Genes (Mus musculus)
Mmp2  (matrix metallopeptidase 2)
Mmp9  (matrix metallopeptidase 9)
Timp4  (tissue inhibitor of metalloproteinase 4)

Genes (Homo sapiens)
MMP2  (matrix metallopeptidase 2)
MMP9  (matrix metallopeptidase 9)
TIMP4  (TIMP metallopeptidase inhibitor 4)


Additional Information