RGD Reference Report - Differential expression of voltage-gated K(+) channel genes in arteries from spontaneously hypertensive and Wistar-Kyoto rats. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources
Advanced Search (OLGA)

Differential expression of voltage-gated K(+) channel genes in arteries from spontaneously hypertensive and Wistar-Kyoto rats.

Authors: Cox, RH  Folander, K  Swanson, R 
Citation: Cox RH, etal., Hypertension. 2001 May;37(5):1315-22.
RGD ID: 1627659
Pubmed: PMID:11358947   (View Abstract at PubMed)

Voltage-gated K(+) currents play an important role in determining membrane potential, intracellular Ca(2+), and contraction in arterial smooth muscle. In this study, the expression of genes encoding voltage-gated K(+) channels of the Kv1.X family was compared in arteries from spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY). Expression of Kv1.X in thoracic aorta, mesenteric arteries, tail artery, and heart was determined, both qualitatively and quantitatively, by reverse transcription-polymerase chain reaction. Our results demonstrate distinct but overlapping patterns of expression in vascular tissues. In general, Kv1.2 and Kv1.5 were most highly represented, and the levels of Kv1.2 were significantly larger in all tissues from SHR. Levels of Kv1.5 in arteries did not differ significantly between strains but were greater in SHR heart. Moderate levels of Kv1.3 and Kvbeta1.1 expression were also found in all tissues and were larger in SHR. Kv1.1 expression was not different between the 2 strains, and no significant expression of Kv1.4 (except in heart and aorta), Kv1.6, or Kvbeta2.1 was observed in either strain. Kv1.2 and Kv1.5 transcripts represent approximately 1 to 2 parts/10(5) of total mesenteric arterial RNA with approximately 2- to 5-fold lower levels in aorta and tail artery. Whole-cell voltage-gated K(+) channel currents, recorded from mesenteric arterial myocytes, were larger in SHR than WKY (eg, at 0 mV: 7.3+/-0.8 versus 10.9+/-1.2 pA/pF). The voltage dependence of activation was more negative in SHR (V(0.5): -20+/-4 mV versus -32+/-3 mV) but that of availability was not different. These results indicate that Kv1.X genes are differentially expressed between WKY and SHR (especially Kv1.2 and Kvbeta1.1). These differences in gene expression are associated with a greater voltage-gated K(+) channel current density in SHR and shifted voltage-dependent activation compared with WKY. These differences may be a compensatory mechanism related to the membrane potential depolarization in SHR or some manifestation thereof.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
KCNA5Humanhypertension  ISOKcna5 (Rattus norvegicus)mRNA:increased expression:heartRGD 
KCNAB1Humanhypertension  ISOKcnab1 (Rattus norvegicus)mRNA:increased expression:aorta and arteries (rat)RGD 
Kcna5Rathypertension  IEP mRNA:increased expression:heartRGD 
Kcna5Mousehypertension  ISOKcna5 (Rattus norvegicus)mRNA:increased expression:heartRGD 
Kcnab1Rathypertension  IEP mRNA:increased expression:aorta and arteries (rat)RGD 
Kcnab1Mousehypertension  ISOKcnab1 (Rattus norvegicus)mRNA:increased expression:aorta and arteries (rat)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Kcna5  (potassium voltage-gated channel subfamily A member 5)
Kcnab1  (potassium voltage-gated channel subfamily A regulatory beta subunit 1)

Genes (Mus musculus)
Kcna5  (potassium voltage-gated channel, shaker-related subfamily, member 5)
Kcnab1  (potassium voltage-gated channel, shaker-related subfamily, beta member 1)

Genes (Homo sapiens)
KCNA5  (potassium voltage-gated channel subfamily A member 5)
KCNAB1  (potassium voltage-gated channel subfamily A regulatory beta subunit 1)


Additional Information