RGD Reference Report - In vitro expression of rat lens aldose reductase in Escherichia coli. - Rat Genome Database

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In vitro expression of rat lens aldose reductase in Escherichia coli.

Authors: Old, SE  Sato, S  Kador, PF  Carper, DA 
Citation: Old SE, etal., Proc Natl Acad Sci U S A. 1990 Jul;87(13):4942-5.
RGD ID: 1626085
Pubmed: PMID:2114645   (View Abstract at PubMed)
PMCID: PMC54237   (View Article at PubMed Central)

Aldose reductase (alditol:NADP+ oxidoreductase, EC 1.1.1.21), an enzyme that converts glucose to sorbitol, the first step of the polyol pathway, has been implicated in secondary complications of diabetes, such as cataracts, retinopathy, neuropathy, and nephropathy. Aldose reductase inhibitors have been observed to prevent or delay the onset of these complications; however, more potent and specific inhibitors are needed. Development of new inhibitors necessitates a better understanding of the molecular structure of this protein. To elucidate the structure-function relationships of aldose reductase and to develop methods of regulating this enzyme, large and homogeneous quantities of rat lens aldose reductase have been expressed in bacterial cells. A construction of the complete coding sequence and 3' untranslated region for rat lens aldose reductase was assembled in the expression vector pKK233-2 (Pharmacia). This construction expresses an active enzyme that has been purified and demonstrates kinetic, immunological, and inhibitory properties similar to rat lens aldose reductase.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Akr1b1Rataldose reductase (NADPH) activity enablesIDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Akr1b1  (aldo-keto reductase family 1 member B1)


Additional Information