RGD Reference Report - Identification of two types of ATP-sensitive K+ channels in rat ventricular myocytes. - Rat Genome Database

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Identification of two types of ATP-sensitive K+ channels in rat ventricular myocytes.

Authors: Wu, SN  Wu, AZ  Sung, RJ 
Citation: Wu SN, etal., Life Sci. 2007 Jan 2;80(4):378-87. Epub 2006 Oct 17.
RGD ID: 1625274
Pubmed: PMID:17097686   (View Abstract at PubMed)
DOI: DOI:10.1016/j.lfs.2006.09.042   (Journal Full-text)

The ATP-sensitive K(+) (K(ATP)) channels are known to provide a functional linkage between the electrical activity of the cell membrane and metabolism. Two types of inwardly rectifying K(+) channel subunits (i.e., Kir6.1 and Kir6.2) with which sulfonylurea receptors are associated were reported to constitute the K(ATP) channels. In this study, we provide evidence to show two types of K(ATP) channels with different biophysical properties functionally expressed in isolated rat ventricular myocytes. Using patch-clamp technique, we found that single-channel conductance for the different two types of K(ATP) channels in these cells was 57 and 21 pS. The kinetic properties, including mean open time and bursting kinetics, did not differ between these two types of K(ATP) channels. Diazoxide only activated the small-conductance K(ATP) channel, while pinacidil and dinitrophenol stimulated both channels. Both of these K(ATP) channels were sensitive to block by glibenclamide. Additionally, western blotting, immunochemistry, and RT-PCR revealed two types of Kir6.X channels, i.e., Kir6.1 and Kir6.2, in rat ventricular myocytes. Single-cell Ca(2+) imaging also revealed that similar to dinitrophenol, diazoxide reduced the concentration of intracellular Ca(2+). The present results suggest that these two types of K(ATP) channels may functionally be related to the activity of heart cells.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Kcnj11Ratpotassium ion transport  IDA  RGD 
Kcnj8Ratpotassium ion transport  IDA  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Kcnj11Ratinward rectifying potassium channel  IDA  RGD 
Kcnj8Ratinward rectifying potassium channel  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Kcnj11RatATP-activated inward rectifier potassium channel activity  IDA  RGD 
Kcnj8RatATP-activated inward rectifier potassium channel activity  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Kcnj11  (potassium inwardly-rectifying channel, subfamily J, member 11)
Kcnj8  (potassium inwardly-rectifying channel, subfamily J, member 8)


Additional Information