RGD Reference Report - Synergy of fatty acid and reactive alkenal activation of proton conductance through uncoupling protein 1 in mitochondria. - Rat Genome Database

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Synergy of fatty acid and reactive alkenal activation of proton conductance through uncoupling protein 1 in mitochondria.

Authors: Esteves, TC  Parker, N  Brand, MD 
Citation: Esteves TC, etal., Biochem J. 2006 May 1;395(3):619-28.
RGD ID: 1624981
Pubmed: PMID:16451125   (View Abstract at PubMed)
PMCID: PMC1462701   (View Article at PubMed Central)
DOI: DOI:10.1042/BJ20052004   (Journal Full-text)

The kinetics of proton transport through mammalian UCP1 (uncoupling protein 1) expressed in yeast mitochondria were measured. There was little or no UCP1 activity in the absence of added palmitate, but significant activity in its presence. The activator 4-HNE (4-hydroxy-2-nonenal) had little effect when added alone, but significantly enhanced proton conductance in the presence of added palmitate. Activation of the proton conductance of UCP1 was synergistic: proton conductance in the presence of both palmitate and 4-HNE was significantly greater than the sum of the individual effects. Mitochondria from control yeast transformed with empty vector showed no such synergy, showing that synergy is a property of UCP1. Activation by the 4-HNE analogue trans-cinnamate showed essentially the same characteristics as activation by 4-HNE. Mitochondria from brown adipose tissue also showed synergistic activation of GDP-sensitive proton conductance by palmitate and 4-HNE. These results show that reactive alkenals activate the proton conductance of UCP1 more strongly when fatty acids are also added, with implications for both mechanistic and physiological models of UCP1 activation.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ucp1Ratpositive regulation of metabolic process  TAS  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ucp1Ratoxidative phosphorylation uncoupler activity  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ucp1  (uncoupling protein 1)


Additional Information