RGD Reference Report - Activation of dolichol-phosphate mannosyltransferase by dibutryl cyclic AMP in rat liver. - Rat Genome Database

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Activation of dolichol-phosphate mannosyltransferase by dibutryl cyclic AMP in rat liver.

Authors: Sharma, C  Radhakrishnamurthy, B  Berenson, GS 
Citation: Sharma C, etal., Biochem Biophys Res Commun. 1988 Sep 15;155(2):615-21.
RGD ID: 1601442
Pubmed: PMID:2844175   (View Abstract at PubMed)

Radiolabeled mannose incorporation into secretory glycoproteins and immunoprecipitable fibronectin in the incubation media significantly increased (105 and 32 percent respectively) with a corresponding increase in the levels of dolichol-phosphate mannose, dolichol-diphosphate oligosaccharides and dolichol-phosphate mannosyltransferase activity in the rat liver slices when incubated with dibutryl cAMP and ATP. Dibutryl cAMP activated maximally this enzyme in the presence of ATP in the incubation medium. The activation of the enzyme resulted in a two fold increase in Vmax with no apparent change in the Km for GDP mannose. Phosphorylation the rat liver microsomes with catalytic subunit of cAMP dependent protein kinase, resulted in the activation of dolichol-phosphate mannosyltransferase. These results suggest that cAMP modulates protein glycosylation by activating dolicholphosphate mannosyltransferase activity. The activation of this enzyme could be through phosphorylation/dephosphorylation mechanism involving a cAMP dependent protein kinase.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Dpm1Ratdolichol metabolic process  IDA  RGD 
Dpm1RatGDP-mannose metabolic process  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Dpm1Ratalcohol binding  IDA  RGD 
Dpm1RatD-mannose binding  IDA  RGD 
Dpm1Ratdolichyl-phosphate beta-D-mannosyltransferase activity  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Dpm1  (dolichyl-phosphate mannosyltransferase subunit 1, catalytic)


Additional Information