RGD Reference Report - Characterizing a rat Brca2 knockout model. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources
Advanced Search (OLGA)

Characterizing a rat Brca2 knockout model.

Authors: Cotroneo, MS  Haag, JD  Zan, Y  Lopez, CC  Thuwajit, P  Petukhova, GV  Camerini-Otero, RD  Gendron-Fitzpatrick, A  Griep, AE  Murphy, CJ  Dubielzig, RR  Gould, MN 
Citation: Cotroneo MS, etal., Oncogene. 2006 Sep 11;.
RGD ID: 1599505
Pubmed: PMID:16964288   (View Abstract at PubMed)
DOI: DOI:10.1038/sj.onc.1209960   (Journal Full-text)

Evidence exists that BRCA2 carriers may have an elevated risk of breast, ovarian, colon, prostate, and pancreatic cancer. In general, carriers are defined as individuals with protein truncating mutations within the BRCA2 gene. Many Brca2 knockout lines have been produced and characterized in the mouse. We previously produced a rat Brca2 knockout strain in which there is a nonsense mutation in exon 11 between BRC repeats 2 and 3, and a truncated protein is produced. Interestingly, while such a mutation in homozygous mice would lead to limited survival of approximately 3 months, the Brca2(-/-) rats are 100% viable and the vast majority live to over 1 year of age. Brca2(-/-) rats show a phenotype of growth inhibition and sterility in both sexes. Aspermatogenesis in the Brca2(-/-) rats is due to a failure of homologous chromosome synapsis. Long-term phenotypes include underdeveloped mammary glands, cataract formation and lifespan shortening due to the development of tumors and cancers in multiple organs. The establishment of the rat Brca2 knockout model provides a means to study the role of Brca2 in increasing cancer susceptibility and inducing a novel ocular phenotype not previously associated with this gene.Oncogene advance online publication, 11 September 2006; doi:10.1038/sj.onc.1209960.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
BRCA2Humancancer  ISOBrca2 (Rattus norvegicus) RGD 
Brca2Ratcancer  IMP  RGD 
Brca2Mousecancer  ISOBrca2 (Rattus norvegicus) RGD 
Brca2m1UwmRatcancer  IMP  RGD 
SD-Brca2m1UwmRatcancer  IMP  RGD 
BRCA2Humancataract  ISOBrca2 (Rattus norvegicus)DNA:nonsense mutationRGD 
Brca2Ratcataract  IAGP DNA:nonsense mutationRGD 
Brca2Ratcataract  IMP  RGD 
Brca2Mousecataract  ISOBrca2 (Rattus norvegicus)DNA:nonsense mutationRGD 
Brca2m1UwmRatcataract  IMP  RGD 
SD-Brca2m1UwmRatcataract  IMP  RGD 
BRCA2Humanosteosarcoma  ISOBrca2 (Rattus norvegicus)DNA:nonsense mutationRGD 
Brca2Ratosteosarcoma  IAGP DNA:nonsense mutationRGD 
Brca2Mouseosteosarcoma  ISOBrca2 (Rattus norvegicus)DNA:nonsense mutationRGD 
BRCA2HumanOvarian Neoplasms susceptibilityISOBrca2 (Rattus norvegicus) RGD 
Brca2RatOvarian Neoplasms susceptibilityIAGP  RGD 
Brca2MouseOvarian Neoplasms susceptibilityISOBrca2 (Rattus norvegicus) RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Brca2Rathomologous chromosome orientation in meiotic metaphase I  IMP  RGD 
Brca2Ratmammary gland development  IMP  RGD 
Brca2Ratmulticellular organism growth  IMP  RGD 
Brca2Ratspermatogenesis  IMP  RGD 

Phenotype Annotations    Click to see Annotation Detail View
Objects Annotated

Genes (Rattus norvegicus)
Brca2  (BRCA2, DNA repair associated)
Brca2m1Uwm  (BRCA2, DNA repair associated; mutation 1, University of Wisconsin-Madison)

Genes (Mus musculus)
Brca2  (breast cancer 2, early onset)

Genes (Homo sapiens)
BRCA2  (BRCA2 DNA repair associated)

Strains
SD-Brca2m1Uwm  (NA)


Additional Information