RGD Reference Report - Hyperammonemia: regulation of argininosuccinate synthetase and argininosuccinate lyase genes in aggregating cell cultures of fetal rat brain. - Rat Genome Database

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Hyperammonemia: regulation of argininosuccinate synthetase and argininosuccinate lyase genes in aggregating cell cultures of fetal rat brain.

Authors: Braissant, O  Honegger, P  Loup, M  Iwase, K  Takiguchi, M  Bachmann, C 
Citation: Braissant O, etal., Neurosci Lett. 1999 May 7;266(2):89-92.
RGD ID: 1599267
Pubmed: PMID:10353334   (View Abstract at PubMed)

Hyperammonemia in the brain leads to poorly understood alterations of nitric oxide (NO) synthesis. Arginine, the substrate of nitric oxide synthases, might be recycled from the citrulline produced with NO by argininosuccinate synthetase (AS) and argininosuccinate lyase (AL). The regulation of AS and AL genes during hyperammonemia is unknown in the brain. We used brain cell aggregates cultured from dissociated telencephalic cortex of rat embryos to analyze the regulation of AS and AL genes in hyperammonemia. Using RNase protection assay and non-radioactive in situ hybridization on aggregate cryosections, we show that both AS and AL genes are induced in astrocytes but not in neurons of aggregates exposed to 5 mM NH4Cl. Our work suggests that the hyperammonemic brain might increase its recycling of citrulline to arginine.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
ASS1HumanHyperammonemia  ISOAss1 (Rattus norvegicus) RGD 
Ass1RatHyperammonemia  IEP  RGD 
Ass1MouseHyperammonemia  ISOAss1 (Rattus norvegicus) RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
AslRatcellular response to ammonium ion  IEP  RGD 
Ass1Ratcellular response to ammonium ion  IEP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Asl  (argininosuccinate lyase)
Ass1  (argininosuccinate synthase 1)

Genes (Mus musculus)
Ass1  (argininosuccinate synthetase 1)

Genes (Homo sapiens)
ASS1  (argininosuccinate synthase 1)

Objects referenced in this article
Gene ASL argininosuccinate lyase Homo sapiens
Gene Asl argininosuccinate lyase Mus musculus

Additional Information