RGD Reference Report - Functional interaction between T2R taste receptors and G-protein alpha subunits expressed in taste receptor cells. - Rat Genome Database

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Functional interaction between T2R taste receptors and G-protein alpha subunits expressed in taste receptor cells.

Authors: Ueda, T  Ugawa, S  Yamamura, H  Imaizumi, Y  Shimada, S 
Citation: Ueda T, etal., J Neurosci. 2003 Aug 13;23(19):7376-80.
RGD ID: 1599039
Pubmed: PMID:12917372   (View Abstract at PubMed)
PMCID: PMC6740443   (View Article at PubMed Central)

Bitter taste perception is a conserved chemical sense against the ingestion of poisonous substances in mammals. A multigene family of G-protein-coupled receptors, T2R (so-called TAS2R or TRB) receptors and a G-protein alpha subunit (Galpha), gustducin, are believed to be key molecules for its perception, but little is known about the molecular basis for its interaction. Here, we use a heterologous expression system to determine a specific domain of gustducin necessary for T2R coupling. Two chimeric Galpha16 proteins harboring 37 and 44 gustducin-specific sequences at their C termini (G16/gust37 and G16/gust44) responded to different T2R receptors with known ligands, but G16/gust 23, G16/gust11, and G16/gust5 did not. The former two chimeras contained a predicted beta6 sheet, an alpha5 helix, and an extreme C terminus of gustducin, and all the domains were indispensable to the expression of T2R activity. We also expressed G16 protein chimeras with the corresponding domain from other Galpha(i) proteins, cone-transducin (Galpha(t2)), Galpha(i2), and Galpha(z) (G16/t2, G16/i2, and G16/z). As a result, G16/t2 and G16/i2 produced specific responses of T2Rs, but G16/z did not. Because Galpha(t2) and Galpha(i2) are expressed in the taste receptor cells, these G-protein alpha(i) subunits may also be involved in bitter taste perception via T2R receptors. The present Galpha16-based chimeras could be useful tools to analyze the functions of many orphan G-protein-coupled taste receptors.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  

Objects Annotated

Genes (Rattus norvegicus)
Gnat2  (G protein subunit alpha transducin 2)


Additional Information