RGD Reference Report - Coupling of bitter receptor to phosphodiesterase through transducin in taste receptor cells. - Rat Genome Database

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Coupling of bitter receptor to phosphodiesterase through transducin in taste receptor cells.

Authors: Ruiz-Avila, L  McLaughlin, SK  Wildman, D  McKinnon, PJ  Robichon, A  Spickofsky, N  Margolskee, RF 
Citation: Ruiz-Avila L, etal., Nature. 1995 Jul 6;376(6535):80-5.
RGD ID: 1599025
Pubmed: PMID:7596440   (View Abstract at PubMed)
DOI: DOI:10.1038/376080a0   (Journal Full-text)

The rod and cone transducins are specific G proteins originally thought to be present only in photoreceptor cells of the vertebrate retina. Transducins convert light stimulation of photoreceptor opsins into activation of cyclic GMP phosphodiesterase (reviewed in refs. 5-7). A transducin-like G protein, gustducin, has been identified and cloned from rat taste cells. We report here that rod transducin is also present in vertebrate taste cells, where it specifically activates a phosphodiesterase isolated from taste tissue. Furthermore, the bitter compound denatonium in the presence of taste-cell membranes activates transducin but not Gi. A peptide that competitively inhibits rhodopsin activation of transducin also blocks taste-cell membrane activation of transducin, arguing for the involvement of a seven-transmembrane-helix G-protein-coupled receptor. These results suggest that rod transducin transduces bitter taste by coupling taste receptor(s) to taste-cell phosphodiesterase. Phosphodieterase-mediated degradation of cyclic nucleotides may lead to taste-cell depolarization through the recently identified cyclic-nucleotide-suppressible conductance.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Gnat1Ratdetection of chemical stimulus involved in sensory perception of bitter taste  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Gnat1RatGTP binding  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Gnat1  (G protein subunit alpha transducin 1)


Additional Information