RGD Reference Report - Structural insights into the functional interaction of KChIP1 with Shal-type K(+) channels. - Rat Genome Database

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Structural insights into the functional interaction of KChIP1 with Shal-type K(+) channels.

Authors: Zhou, W  Qian, Y  Kunjilwar, K  Pfaffinger, PJ  Choe, S 
Citation: Zhou W, etal., Neuron. 2004 Feb 19;41(4):573-86.
RGD ID: 1581450
Pubmed: PMID:14980206   (View Abstract at PubMed)

Four Kv channel-interacting proteins (KChIP1 through KChIP4) interact directly with the N-terminal domain of three Shal-type voltage-gated potassium channels (Kv4.1, Kv4.2, and Kv4.3) to modulate cell surface expression and function of Kv4 channels. Here we report a 2.0 Angstrom crystal structure of the core domain of KChIP1 (KChIP1*) in complex with the N-terminal fragment of Kv4.2 (Kv4.2N30). The complex reveals a clam-shaped dimeric assembly. Four EF-hands from each KChIP1 form each shell of the clam. The N-terminal end of Kv4.2 forming an alpha helix (alpha1) and the C-terminal alpha helix (H10) of KChIP1 are enclosed nearly coaxially by these shells. As a result, the H10 of KChIP1 and alpha1 of Kv4.2 mediate interactions between these two molecules, structurally reminiscent of the interactions between calmodulin and its target peptides. Site-specific mutagenesis combined with functional characterization shows that those interactions mediated by alpha1 and H10 are essential to the modulation of Kv4.2 by KChIPs.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Kcnip1Ratpositive regulation of action potential  IDA  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Kcnd2Ratpotassium channel complex  IDA  RGD 
Kcnip1Ratpotassium channel complex  IDA  RGD 

Molecular Function

  

Objects Annotated

Genes (Rattus norvegicus)
Kcnd2  (potassium voltage-gated channel subfamily D member 2)
Kcnip1  (potassium voltage-gated channel interacting protein 1)


Additional Information