RGD Reference Report - Proteomic analysis of rat liver peroxisome: presence of peroxisome-specific isozyme of Lon protease. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources
Advanced Search (OLGA)

Proteomic analysis of rat liver peroxisome: presence of peroxisome-specific isozyme of Lon protease.

Authors: Kikuchi, M  Hatano, N  Yokota, S  Shimozawa, N  Imanaka, T  Taniguchi, H 
Citation: Kikuchi M, etal., J Biol Chem. 2004 Jan 2;279(1):421-8. Epub 2003 Oct 15.
RGD ID: 1580664
Pubmed: PMID:14561759   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M305623200   (Journal Full-text)

Subcellular proteomics, which includes isolation of subcellular components prior to a proteomic analysis, is advantageous not only in characterizing large macro-molecular complexes such as organelles but also in elucidating mechanisms of protein transport and organelle biosynthesis. Because of the high sensitivity achieved by the present proteomics technology, the purity of samples to be analyzed is important for the interpretation of the results obtained. In the present study, peroxisomes isolated from rat liver by usual cell fractionation were further purified by immunoisolation using a specific antibody raised against a peroxisomal membrane protein, PMP70. The isolated peroxisomes were analyzed by SDS-PAGE combined with liquid chromatography/mass spectrometry. Altogether 34 known peroxisomal proteins were identified in addition to several mitochondrial and microsomal proteins. Some of the latter may reside in the peroxisomes as well. Analysis of membrane fractions identified all known peroxins except for Pex7. Two new peroxisomal proteins of unknown function were of high abundance. One is a bi-functional protein consisting of an aminoglycoside phosphotransferase-domain and an acyl-CoA dehydrogenase domain. The other is a newly identified peroxisome-specific isoform of Lon protease, an ATP-dependent protease with chaperone-like activity. The peroxisomal localization of the protein was confirmed by immunological techniques. The peroxisome-type Lon protease, which is distinct from the mitochondrial isoform, may play an important role in the peroxisomal biogenesis.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Aldh3a2Ratendoplasmic reticulum located_inIDA PMID:14561759HGNC-UCL 
Cyb5aRatendoplasmic reticulum located_inIDA PMID:14561759HGNC-UCL 
Mgst1Ratendoplasmic reticulum located_inIDA PMID:14561759HGNC-UCL 
Ugt1a1Ratendoplasmic reticulum located_inIDA PMID:14561759HGNC-UCL 
Pdia6Ratendoplasmic reticulum lumen located_inIDA PMID:14561759HGNC-UCL 
Cyb5r3Ratendoplasmic reticulum membrane located_inIDA PMID:14561759HGNC-UCL 
Rab2aRatmembrane located_inIDA PMID:14561759BHF-UCL 
VapaRatmembrane located_inIDA PMID:14561759BHF-UCL 
AgxtRatmitochondrial matrix located_inIDA PMID:14561759HGNC-UCL 
Gstk1Ratmitochondrial matrix located_inIDA PMID:14561759HGNC-UCL 
Cps1Ratmitochondrion located_inIDA PMID:14561759HGNC-UCL 
Prdx5Ratmitochondrion located_inIDA PMID:14561759HGNC-UCL 
Lonp2Ratperoxisomal matrix  IDA  RGD 
Lonp2Ratperoxisomal matrix located_inIDA PMID:14561759HGNC-UCL 
Abcd2Ratperoxisomal membrane located_inIDA PMID:14561759HGNC-UCL 
Abcd3Ratperoxisomal membrane located_inIDA PMID:14561759HGNC-UCL 
Acsl1Ratperoxisomal membrane located_inIDA PMID:14561759HGNC-UCL 
Pex1Ratperoxisomal membrane  IDA  RGD 
Pex10Ratperoxisomal membrane  IDA  RGD 
Pex11aRatperoxisomal membrane located_inIDA PMID:14561759HGNC-UCL 
Pex11aRatperoxisomal membrane  IDA  RGD 
Pex11bRatperoxisomal membrane  IDA  RGD 
Pex12Ratperoxisomal membrane located_inIDA PMID:14561759HGNC-UCL 
Pex12Ratperoxisomal membrane  IDA  RGD 
Pex13Ratperoxisomal membrane located_inIDA PMID:14561759BHF-UCL 
Pex13Ratperoxisomal membrane  IDA  RGD 
Pex14Ratperoxisomal membrane  IDA  RGD 
Pex14Ratperoxisomal membrane located_inIDA PMID:14561759HGNC-UCL 
Pex16Ratperoxisomal membrane  IDA  RGD 
Pex2Ratperoxisomal membrane  IDA  RGD 
Pex2Ratperoxisomal membrane located_inIDA PMID:14561759HGNC-UCL 
Pex3Ratperoxisomal membrane  IDA  RGD 
Pex3Ratperoxisomal membrane located_inIDA PMID:14561759HGNC-UCL 
Pex5Ratperoxisomal membrane located_inIDA PMID:14561759HGNC-UCL 
Pex5Ratperoxisomal membrane  IDA  RGD 
Pex6Ratperoxisomal membrane located_inIDA PMID:14561759HGNC-UCL 
Pex6Ratperoxisomal membrane  IDA  RGD 
Pxmp2Ratperoxisomal membrane located_inIDA PMID:14561759HGNC-UCL 
Pxmp4Ratperoxisomal membrane located_inIDA PMID:14561759HGNC-UCL 
Slc27a2Ratperoxisomal membrane located_inIDA PMID:14561759HGNC-UCL 
Acaa1aRatperoxisome located_inIDA PMID:14561759HGNC-UCL 
Acad11Ratperoxisome located_inIDA PMID:14561759HGNC-UCL 
Acox2Ratperoxisome located_inIDA PMID:14561759HGNC-UCL 
Acox3Ratperoxisome located_inIDA PMID:14561759HGNC-UCL 
AgpsRatperoxisome located_inIDA PMID:14561759HGNC-UCL 
AgxtRatperoxisome located_inIDA PMID:14561759HGNC-UCL 
AmacrRatperoxisome located_inIDA PMID:14561759HGNC-UCL 
BaatRatperoxisome located_inIDA PMID:14561759HGNC-UCL 
CatRatperoxisome located_inIDA PMID:14561759HGNC-UCL 
Decr2Ratperoxisome located_inIDA PMID:14561759HGNC-UCL 
EhhadhRatperoxisome located_inIDA PMID:14561759HGNC-UCL 
Fis1Ratperoxisome located_inIDA PMID:14561759HGNC-UCL 
GnpatRatperoxisome located_inIDA PMID:14561759HGNC-UCL 
Hao2Ratperoxisome located_inIDA PMID:14561759HGNC-UCL 
Hsd17b4Ratperoxisome located_inIDA PMID:14561759HGNC-UCL 
Idh1Ratperoxisome located_inIDA PMID:14561759HGNC-UCL 
Isoc1Ratperoxisome located_inIDA PMID:14561759HGNC-UCL 
Lonp2Ratperoxisome  IDA  RGD 
PecrRatperoxisome located_inIDA PMID:14561759HGNC-UCL 
Pex14Ratperoxisome located_inIDA PMID:14561759HGNC-UCL 
Pex2Ratperoxisome located_inIDA PMID:14561759HGNC-UCL 
Pex3Ratperoxisome located_inIDA PMID:14561759HGNC-UCL 
PhyhRatperoxisome located_inIDA PMID:14561759HGNC-UCL 
Prdx5Ratperoxisome located_inIDA PMID:14561759HGNC-UCL 
Scp2Ratperoxisome located_inIDA PMID:14561759HGNC-UCL 
UoxRatperoxisome located_inIDA PMID:14561759HGNC-UCL 

Objects Annotated

Genes (Rattus norvegicus)
Abcd2  (ATP binding cassette subfamily D member 2)
Abcd3  (ATP binding cassette subfamily D member 3)
Acaa1a  (acetyl-CoA acyltransferase 1A)
Acad11  (acyl-CoA dehydrogenase family, member 11)
Acox2  (acyl-CoA oxidase 2)
Acox3  (acyl-CoA oxidase 3, pristanoyl)
Acsl1  (acyl-CoA synthetase long-chain family member 1)
Agps  (alkylglycerone phosphate synthase)
Agxt  (alanine--glyoxylate aminotransferase)
Aldh3a2  (aldehyde dehydrogenase 3 family, member A2)
Amacr  (alpha-methylacyl-CoA racemase)
Baat  (bile acid CoA:amino acid N-acyltransferase)
Cat  (catalase)
Cps1  (carbamoyl-phosphate synthase 1)
Cyb5a  (cytochrome b5 type A)
Cyb5r3  (cytochrome b5 reductase 3)
Decr2  (2,4-dienoyl-CoA reductase 2)
Ehhadh  (enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase)
Fis1  (fission, mitochondrial 1)
Gnpat  (glyceronephosphate O-acyltransferase)
Gstk1  (glutathione S-transferase kappa 1)
Hao2  (hydroxyacid oxidase 2)
Hsd17b4  (hydroxysteroid (17-beta) dehydrogenase 4)
Idh1  (isocitrate dehydrogenase (NADP(+)) 1)
Isoc1  (isochorismatase domain containing 1)
Lonp2  (lon peptidase 2, peroxisomal)
Mgst1  (microsomal glutathione S-transferase 1)
Pdia6  (protein disulfide isomerase family A, member 6)
Pecr  (peroxisomal trans-2-enoyl-CoA reductase)
Pex1  (peroxisomal biogenesis factor 1)
Pex10  (peroxisomal biogenesis factor 10)
Pex11a  (peroxisomal biogenesis factor 11 alpha)
Pex11b  (peroxisomal biogenesis factor 11 beta)
Pex12  (peroxisomal biogenesis factor 12)
Pex13  (peroxisomal biogenesis factor 13)
Pex14  (peroxisomal biogenesis factor 14)
Pex16  (peroxisomal biogenesis factor 16)
Pex2  (peroxisomal biogenesis factor 2)
Pex3  (peroxisomal biogenesis factor 3)
Pex5  (peroxisomal biogenesis factor 5)
Pex6  (peroxisomal biogenesis factor 6)
Phyh  (phytanoyl-CoA 2-hydroxylase)
Prdx5  (peroxiredoxin 5)
Pxmp2  (peroxisomal membrane protein 2)
Pxmp4  (peroxisomal membrane protein 4)
Rab2a  (RAB2A, member RAS oncogene family)
Scp2  (sterol carrier protein 2)
Slc27a2  (solute carrier family 27 member 2)
Ugt1a1  (UDP glucuronosyltransferase family 1 member A1)
Uox  (urate oxidase)
Vapa  (VAMP associated protein A)

Objects referenced in this article
Gene Lonp1 lon peptidase 1, mitochondrial Rattus norvegicus

Additional Information