RGD Reference Report - Nitric oxide synthase localization in the rat neutrophils: immunocytochemical, molecular, and biochemical studies. - Rat Genome Database

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Nitric oxide synthase localization in the rat neutrophils: immunocytochemical, molecular, and biochemical studies.

Authors: Saini, R  Patel, S  Saluja, R  Sahasrabuddhe, AA  Singh, MP  Habib, S  Bajpai, VK  Dikshit, M 
Citation: Saini R, etal., J Leukoc Biol. 2006 Mar;79(3):519-28. Epub 2005 Dec 30.
RGD ID: 1580263
Pubmed: PMID:16387842   (View Abstract at PubMed)
DOI: DOI:10.1189/jlb.0605320   (Journal Full-text)

Nitric oxide (NO) modulates diverse functions of polymorphonuclear neutrophils (PMNs), but localization of NO synthase (NOS) and identification of its interacting proteins remain the least defined. The present study discerns subcellular distribution of NOS and caveolin-1, a prominent NOS-interacting protein in rat PMNs. Localization of NOS was explored by confocal and immunogold electron microscopy, and its activity was assessed by L-[3H] arginine and 4,5-diaminofluorescein diacetate (DAF-2DA). Reverse transcriptase-polymerase chain reaction using NOS primers and Western blotting demonstrated the presence of neuronal NOS (nNOS) and inducible NOS (iNOS) in PMNs. Immunocytochemical studies exhibited distribution of nNOS and iNOS in cytoplasm and nucleus, and L-[3H] citrulline formation and DAF fluorescence confirmed NOS activity in both fractions. NOS activity correlated positively with calmodulin concentration in both of the fractions. nNOS and iNOS colocalized with caveolin-1, as evidenced by immunocytochemical and immunoprecipitation studies. The results thus provide first evidence of nNOS and iNOS in the nuclear compartment and suggest NOS interaction with caveolin-1 in rat PMNs.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Nos1Ratazurophil granule  IDA  RGD 
Nos1Ratnuclear membrane  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Nos1Ratnitric-oxide synthase activity  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Nos1  (nitric oxide synthase 1)

Objects referenced in this article
Gene Nos2 nitric oxide synthase 2 Rattus norvegicus

Additional Information