RGD Reference Report - Genetic linkage of urinary albumin excretion in Dahl salt-sensitive rats: influence of dietary salt and confirmation using congenic strains. - Rat Genome Database

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Genetic linkage of urinary albumin excretion in Dahl salt-sensitive rats: influence of dietary salt and confirmation using congenic strains.

Authors: Garrett, MR  Joe, B  Yerga-Woolwine, S 
Citation: Garrett MR, etal., Physiol Genomics. 2006 Mar 13;25(1):39-49.
RGD ID: 1578520
Pubmed: (View Article at PubMed) PMID:16534143
DOI: Full-text: DOI:10.1152/physiolgenomics.00150.2005

Previously, we reported a linkage analysis for urinary albumin excretion (UAE) from a backcross population derived from the Dahl salt-sensitive (S) rat and the spontaneously hypertensive rat (SHR) raised on a low-salt diet. The present study sought to examine the effect of salt loading on the observation of UAE quantitative trait loci (QTL) using a F1(S x SHR) x S backcross population (n = 228) raised on a 2% NaCl diet. Parental strain data demonstrated that S rats have significantly higher blood pressure (BP) and UAE compared with either F1(S x SHR) or SHR at 8 wk of age, and this difference was exacerbated by 12 wk of age in response to a high-salt diet (2% NaCl). Genome scans done at 8, 12, and 16 wk of age yielded eight QTL for UAE. At week 8 (low salt), QTL for UAE were observed on rat chromosomes (RNO) 1, 2, 6, 8, 9, 11, 13, and 19. Week 8 linkage analysis confirmed previous linkage data and provided a baseline to examine the effect of salt loading at subsequent time points. At weeks 12 and 16 (after salt- loading), QTL for UAE were observed on RNO1, -6, -8, -9, and -13. Surprisingly, UAE QTL were no longer observed on RNO2, -11, and -19 after salt loading, suggesting that these QTL are attenuated by increased salt intake. The effects of UAE QTL on RNO2, -6, -9, -11, and -13 were examined using congenic strains whereby the SHR alleles at each QTL were placed on the S background. These congenic strains demonstrated large and significant effects on UAE compared with the S rat, proving that QTL for UAE reside on these chromosomes.

Disease Annotations    
Albuminuria  (IAGP,IDA)
proteinuria  (IAGP,IDA)

Phenotype Annotations    

Mammalian Phenotype
Objects Annotated

Cm52  (Cardiac mass QTL 52)
Cm53  (Cardiac mass QTL 53)
Kidm29  (Kidney mass QTL 29)
Klgr1  (Kidney lesion grade QTL 1)
Pur10  (Proteinuria QTL 10)
Pur11  (Proteinuria QTL 11)
Pur4  (Proteinuria QTL 4)
Pur5  (Proteinuria QTL 5)
Pur6  (Proteinuria QTL 6)
Pur7  (Proteinuria QTL 7)
Pur8  (Proteinuria QTL 8)
Uae30  (Urinary albumin excretion QTL 30)
Uae31  (Urinary albumin excretion QTL 31)
Uae32  (Urinary albumin excretion QTL 32)
Uae33  (Urinary albumin excretion QTL 33)
Uae34  (Urinary albumin excretion QTL 34)
Uae35  (Urinary albumin excretion QTL 35)
Uae36  (Urinary albumin excretion QTL 36)
Uae39  (Urinary albumin excretion QTL 39)

Objects referenced in this article
Strain SS.SHR-(D11Mgh3-D11Rat31)/Mco null Rattus norvegicus
Strain SS.SHR-(D13Rat63-D13Mit1)/Mco null Rattus norvegicus
Strain SS.SHR-(D2Rat61-D2Mco18)/Mco null Rattus norvegicus
Strain SS.SHR-(D6Wox13-D6Rat84)/Mco null Rattus norvegicus
Strain SS.SHR-(D9Wox16-D9Rat64)/Mco null Rattus norvegicus

Additional Information