RGD Reference Report - Enhanced expression of cytosolic fatty acid binding protein and fatty acid uptake during liver regeneration in rats. - Rat Genome Database

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Enhanced expression of cytosolic fatty acid binding protein and fatty acid uptake during liver regeneration in rats.

Authors: Wang, G  Chen, QM  Minuk, GY  Gong, Y  Burczynski, FJ 
Citation: Wang G, etal., Mol Cell Biochem. 2004 Jul;262(1-2):41-9.
RGD ID: 1578454
Pubmed: PMID:15532708   (View Abstract at PubMed)

BACKGROUND/AIMS: Cytoplasmic liver fatty acid binding protein (L-FABP) has been suggested to be associated with cellular mitotic activity but the changes in L-FABP mRNA and protein levels during liver regeneration following partial hepatectomy (PHx) are not clear. METHODS: In the present study, we determined the time course of L-FABP mRNA expression and L-FABP levels following 70% PHx using Northern and Western blot, respectively. To elucidate one of the roles for L-FABP in PHx, [3H]-palmitic acid clearance in hepatocytes isolated from 24 h post-PHx and control animals was assessed. RESULTS: L-FABP mRNA increased at 30 min, peaked at approximately 1 h (163 +/- 17%; mean +/- SE, n = 5), and returned to control levels 6 h post-PHx. L-FABP level also increased at 1 h but peaked at 24-h (219 +/- 41%; mean +/- SE, n = 5). Hepatocyte [3H]-palmitic acid clearance increased by 29% at 24-h post-PHx, suggesting an increased intracellular transport (or binding) function by L-FABP. Pre-treatment with dexamethasone statistically reduced L-FABP levels (29%) and suppressed the regenerative process (mitotic activity). CONCLUSIONS: L-FABP mRNA increased sharply in response to PHx but the increase was short lived, while L-FABP level increased at a later stage. Both L-FABP content and fatty acid uptake increased significantly during liver regeneration induced by PHx in rats. It is likely that L-FABP is one of the factors responsible for hepatic regeneration.

Phenotype Annotations    Click to see Annotation Detail View

Mammalian Phenotype

TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
enhanced liver regeneration  IAGP 1578454 RGD 
Objects Annotated

Genes (Rattus norvegicus)
Fabp1  (fatty acid binding protein 1)


Additional Information