RGD Reference Report - The genomic organization of type I keratin genes in mice. - Rat Genome Database

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The genomic organization of type I keratin genes in mice.

Authors: Sato, H  Koide, T  Sagai, T  Ishiguro, SI  Tamai, M  Saitou, N  Shiroishi, T 
Citation: Sato H, etal., Genomics 1999 Mar 15;56(3):303-9.
RGD ID: 1556673
Pubmed: PMID:10087197   (View Abstract at PubMed)
DOI: DOI:10.1006/geno.1998.5721   (Journal Full-text)

We isolated two new keratin cDNAs by screening a cDNA library constructed from poly(A)+ RNA of the dorsal and abdominal skin of C57BL/10J mice with a probe of human KRT14. Due to its high sequence homology to human keratin 17 cDNA, one full-length cDNA is most likely to be mouse keratin 17 (Krt1-17) cDNA. The other is the putative full-length cDNA of a novel type I keratin gene, designated Krt1-c29. These two keratin genes were mapped to the distal portion of Chromosome 11, where the mouse keratin gene complex-1 (Krt1) is localized. To elucidate the genomic organization of Krt1 in mice, we carried out genetic and physical analyses of Krt1. A large-scale linkage analysis using intersubspecific backcrosses suggested that there are two major clusters in Krt1, one containing Krt1-c29, Krt1-10, and Krt1-12 and the other containing Krt1-14, -15, -17, and -19. Truncation experiments with two yeast artificial chromosome clones containing the two clusters above have revealed that the gene order of Krt1 is centromere-Krt1-c29-Krt1-10-Krt1-12-Krt1-13-K rt1-15-Krt1-19-Krt1-14-K rt1-17-telomere. Finally, we analyzed sequence divergence between the genes belonging to the Krt1 complex. The results clearly indicated that genes are classified into two major groups with respect to phylogenetic relationship. Each group consists of the respective gene cluster demonstrated by genetic and physical analyses in this study, suggesting that the physical organization of the Krt1 complex reflects the evolutionary process of gene duplication of this complex.



Objects referenced in this article
Gene Grn granulin Mus musculus
Gene Jup junction plakoglobin Mus musculus
Gene Krt10 keratin 10 Mus musculus
Gene Krt13 keratin 13 Mus musculus
Gene Krt14 keratin 14 Mus musculus
Gene Krt15 keratin 15 Mus musculus
Gene Krt17 keratin 17 Mus musculus
Gene Krt19 keratin 19 Mus musculus
Gene Krt27 keratin 27 Mus musculus
Gene Krt32 keratin 32 Mus musculus
Gene Krt34 keratin 34 Mus musculus

Additional Information