RGD Reference Report - Fast and selective ammonia transport by aquaporin-8. - Rat Genome Database

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Fast and selective ammonia transport by aquaporin-8.

Authors: Saparov, Sapar M  Liu, Kun  Agre, Peter  Pohl, Peter 
Citation: Saparov SM, etal., J Biol Chem. 2007 Feb 23;282(8):5296-301. doi: 10.1074/jbc.M609343200. Epub 2006 Dec 21.
RGD ID: 155663531
Pubmed: PMID:17189259   (View Abstract at PubMed)
PMCID: PMC3056221   (View Article at PubMed Central)
DOI: DOI:10.1074/jbc.M609343200   (Journal Full-text)

The transport of ammonia/ammonium is fundamental to nitrogen metabolism in all forms of life. So far, no clear picture has emerged as to whether a protein channel is capable of transporting exclusively neutral NH(3) while excluding H(+) and NH(4)(+). Our research is the first stoichiometric study to show the selective transport of NH(3) by a membrane channel. The purified water channel protein aquaporin-8 was reconstituted into planar bilayers, and the exclusion of NH(4)(+) or H(+) was established by ensuring a lack of current under voltage clamp conditions. The single channel water permeability coefficient of 1.2 x 10(-14) cm(3)/subunit/s was established by imposing an osmotic gradient across reconstituted planar bilayers, and resulting minute changes in ionic concentration close to the membrane surface were detected. It is more than 2-fold smaller than the single channel ammonia permeability (2.7 x 10(-14) cm(3)/subunit/s) that was derived by establishing a transmembrane ammonium concentration gradient and measuring the resulting concentration increases adjacent to the membrane. This permeability ratio suggests that electrically silent ammonia transport may be the main function of AQP8.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Aqp8Ratammonium import across plasma membrane involved_inIDA PMID:17189259UniProt 
Aqp8Ratwater transport involved_inIMP PMID:17189259UniProt 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Aqp8Ratammonium channel activity enablesIDA PMID:17189259UniProt 
Aqp8Ratwater channel activity enablesIMP PMID:17189259UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Aqp8  (aquaporin 8)


Additional Information