RGD Reference Report - Spectrins in developing rat hippocampal cells. - Rat Genome Database

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Spectrins in developing rat hippocampal cells.

Authors: Ursitti, J A  Martin, L  Resneck, W G  Chaney, T  Zielke, C  Alger, B E  Bloch, R J 
Citation: Ursitti JA, etal., Brain Res Dev Brain Res. 2001 Jul 23;129(1):81-93. doi: 10.1016/s0165-3806(01)00160-2.
RGD ID: 155230707
Pubmed: PMID:11454415   (View Abstract at PubMed)
DOI: DOI:10.1016/s0165-3806(01)00160-2   (Journal Full-text)

We studied the spectrins in developing hippocampal tissue in vivo and in vitro to learn how they contribute to the organization of synaptic and extrasynaptic regions of the neuronal plasma membrane. beta-Spectrin, but not beta-fodrin or alpha-fodrin, increased substantially during postnatal development in the hippocampus, where it was localized in neurons but not in astrocytes. Immunoprecipitations from neonatal and adult hippocampal extracts suggest that while both beta-spectrin and beta-fodrin form heteromers with alpha-fodrin, oligomers containing all three subunits are also present. At the subcellular level, beta-fodrin and alpha-fodrin were present in the cell bodies, dendrites, and axons of pyramidal-like neurons in culture, as well as in astrocytes. beta-Spectrin, by contrast, was absent from axons but present in cell bodies and dendrites, where it was organized in a loose, membrane-associated meshwork that lacked alpha-fodrin. A similar meshwork was also apparent in pyramidal neurons in vivo. At some dendritic spines, alpha-fodrin was present in the necks but not in the heads, whereas beta-spectrin was present at significant levels in the spine heads. The presence of significant amounts of beta-spectrin without an accompanying alpha-fodrin subunit was confirmed by immunoprecipitations from extracts of adult hippocampus. Our results suggest that the spectrins in hippocampal neurons can assemble to form different membrane-associated structures in distinct membrane domains, including those at synapses.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Sptan1Ratglutamatergic synapse is_active_inIDA PMID:11454415SynGO 
SptbRatglutamatergic synapse is_active_inIDA PMID:11454415SynGO 
Sptan1Ratpostsynapse is_active_inIDA PMID:11454415SynGO 
SptbRatpostsynapse is_active_inIDA PMID:11454415SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Sptan1  (spectrin, alpha, non-erythrocytic 1)
Sptb  (spectrin, beta, erythrocytic)


Additional Information