RGD Reference Report - Genetic analysis of diet-induced hypercholesterolemia in exogenously hypercholesterolemic (ExHC) rats. - Rat Genome Database

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Genetic analysis of diet-induced hypercholesterolemia in exogenously hypercholesterolemic (ExHC) rats.

Authors: Asahina, M  Sato, M  Imaizumi, K 
Citation: Asahina M, et al., J Lipid Res 46(10):2289-2294, 2005.
RGD ID: 1547851
Pubmed: (View Article at PubMed) PMID:16061941
DOI: Full-text: DOI:10.1194/jlr.M500257-JLR200

Exogenously hypercholesterolemic (ExHC) rats are an established strain that exhibits a polygenic syndrome of hypercholesterolemia after feeding on a cholesterol-containing diet, and the extent of this differs between male and female rats in the strain. The present study was performed to determine the genetic background of diet-induced hypercholesterolemia in ExHC rats. We employed quantitative trait locus (QTL) analyses of the F2 progeny derived from ExHC and Brown-Norway rats. Rats were fed a diet containing 1% cholesterol, and a genome-wide scan was then performed. Significant QTLs for serum total cholesterol levels were revealed on chromosomes 5 and 14 in the vicinity of markers D5Rat95 and D14Rat43, having maximum logarithm of odds (LOD) scores of 6.0 and 5.8, respectively. A suggestive QTL for the trait was also detected on chromosome 3 at D3Rat140. In particular, the QTL on chromosome 5 was specific for female rats. These loci were novel QTLs for post-dietary serum total cholesterol levels. In addition, the cross-mating analysis in F1 generations suggested that the responsiveness to dietary cholesterol in ExHC rats is partly due to X-linked inheritance. Identifying such genetic factors may be useful in predicting the risks associated with diet-induced hypercholesterolemia in humans.



Disease Annotations    

Phenotype Annotations    

Mammalian Phenotype

Objects Annotated

QTLs
Scl44  (Serum cholesterol level QTL 44)
Scl45  (Serum cholesterol level QTL 45)
Scl46  (Serum cholesterol level QTL 46)
Scl47  (Serum cholesterol level QTL 47)
Scl48  (Serum cholesterol level QTL 48)

Objects referenced in this article
Strain BN/Seac null Rattus norvegicus
Strain ExHC/Seac null Rattus norvegicus

Additional Information