RGD Reference Report - Electrogenic cotransport of Na+ and sulfate in Xenopus oocytes expressing the cloned Na+SO4(2-) transport protein NaSi-1. - Rat Genome Database

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Electrogenic cotransport of Na+ and sulfate in Xenopus oocytes expressing the cloned Na+SO4(2-) transport protein NaSi-1.

Authors: Busch, A E  Waldegger, S  Herzer, T  Biber, J  Markovich, D  Murer, H  Lang, F 
Citation: Busch AE, etal., J Biol Chem. 1994 Apr 29;269(17):12407-9.
RGD ID: 153298952
Pubmed: PMID:8175644   (View Abstract at PubMed)

The Na+/sulfate cotransporter cloned from rat kidney cortex (NaSi-1) has been expressed in oocytes of Xenopus laevis and subjected to electrophysiological analysis by current and voltage clamp methods. In current-clamped oocytes, superfusion with 1 mM sulfate resulted in a 12-mV depolarization of the cell membrane. Accordingly, in voltage-clamped oocytes sulfate induced an inward current IS, which was dependent on both the concentration of Na+ and sulfate in the superfusate. Half-maximal IS was observed at about 0.1 mM sulfate and 70 mM Na+. The Hill coefficients were 1 and 2.8 for sulfate and Na+, respectively. Thiosulfate and selenate created similar currents as sulfate with a similar Km. At saturating concentrations of thiosulfate and selenate, addition of sulfate could not induce an additive current. Phosphate (1 mM) did not inhibit sulfate-induced currents. Finally, IS was dependent on the holding potential being larger at more negative potentials. The results of this study strongly suggest an electrogenic cotransport of sulfate and Na+ with a stoichiometry of 1:3.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc13a1Ratmonoatomic anion:sodium symporter activity enablesIDA PMID:8175644UniProt 
Slc13a1Ratsodium:sulfate symporter activity enablesIDA PMID:8175644UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Slc13a1  (solute carrier family 13 member 1)


Additional Information