RGD Reference Report - Stable expression of biogenic amine transporters reveals differences in inhibitor sensitivity, kinetics, and ion dependence. - Rat Genome Database

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Stable expression of biogenic amine transporters reveals differences in inhibitor sensitivity, kinetics, and ion dependence.

Authors: Gu, H  Wall, S C  Rudnick, G 
Citation: Gu H, etal., J Biol Chem. 1994 Mar 11;269(10):7124-30.
RGD ID: 152995566
Pubmed: PMID:8125921   (View Abstract at PubMed)

We have constructed stable cell lines expressing transporters for dopamine (DA), norepinephrine (NE), and serotonin (5-HT) by transfection with cloned cDNAs. The parental LLC-PK1 cell does not express any of these neurotransmitter transporters. Therefore, monoamine transport activities in each of these cell lines are due to the transfected DNA only, allowing comparison in the same background. Drug inhibition profiles for each cell line are distinct and as expected for each transporter. LLC-NET and LLC-DAT cells transported both NE and DA and both cell types exhibited a lower KM for DA transport than for NE transport. Analysis of Vmax data for LLC-NET cells suggests that substrate is bound to the NE transporter during the rate-limiting step(s) in transport. The cocaine analog 2-beta-carbomethoxy-3 beta-(4-[125I]iodophenyl)tropane binds to each cell type, and is displaced by transport substrate in each case. Binding and transport measurements on parallel cell cultures allowed estimation of turnover numbers for norepinephrine, dopamine, and serotonin transporters. All three transporters require external Na+ and Cl-. The Na+ concentration dependence suggests that a single Na+ ion is involved in transport catalyzed by norepinephrine and serotonin transporters while more than one Na+ ion participate in transport mediated by the dopamine transporter.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc6a3Ratdopamine:sodium symporter activity enablesIDA PMID:8125921UniProt 
Slc6a3Ratnorepinephrine:sodium symporter activity enablesIDA PMID:8125921UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Slc6a3  (solute carrier family 6 member 3)


Additional Information