RGD Reference Report - Alpha2 nicotine receptors function as a molecular switch to continuously excite a subset of interneurons in rat hippocampal circuits. - Rat Genome Database

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Alpha2 nicotine receptors function as a molecular switch to continuously excite a subset of interneurons in rat hippocampal circuits.

Authors: Jia, Yousheng  Yamazaki, Yoshihiko  Nakauchi, Sakura  Sumikawa, Katumi 
Citation: Jia Y, etal., Eur J Neurosci. 2009 Apr;29(8):1588-603. doi: 10.1111/j.1460-9568.2009.06706.x.
RGD ID: 152995360
Pubmed: PMID:19385992   (View Abstract at PubMed)
PMCID: PMC2915898   (View Article at PubMed Central)
DOI: DOI:10.1111/j.1460-9568.2009.06706.x   (Journal Full-text)

Rapid activation of nicotinic acetylcholine receptors (nAChRs) at various anatomical and cellular locations in the hippocampus differentially modulates the operation of hippocampal circuits. However, it is largely unknown how the continued presence of nicotine affects the normal operation of hippocampal circuits. Here, we used single and dual whole-cell recordings to address this question. We found that horizontally oriented interneurons in the stratum oriens/alveus continuously discharged action potentials in the presence of nicotine. In these interneurons, bath application of nicotine produced slow inward currents that were well maintained and inhibited by the non-alpha 7 antagonist dihydro-beta-erythroidine. Single-cell reverse transcription-polymerase chain reaction analysis showed that nicotine-responding interneurons were consistently positive for the alpha2 subunit mRNA. These observations suggest that in the presence of nicotine, a subset of interneurons in the stratum oriens/alveus are continuously excited due to the sustained activation of alpha2* nAChRs. These interneurons were synaptically connected to pyramidal cells, and nicotine increased inhibitory baseline currents at the synapses and suppressed phasic inhibition at the same synapses. Nicotine-induced inhibitory activity increased background noise and masked small phasic inhibition in pyramidal cells, originating from other interneurons in the stratum radiatum. Thus, the continued presence of nicotine alters the normal operation of hippocampal circuits by gating inhibitory circuits through activating a non-desensitizing alpha2 nAChR subtype on a distinct population of interneurons.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Chrna2Ratcellular response to nicotine  IDA MMO:0000293RGD 
Chrna2Ratmodulation of inhibitory postsynaptic potential  IDA MMO:0000293RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Chrna2Ratneuron projection cytoplasm  IDA MMO:0000293 and MMO:0000656RGD 

Objects Annotated

Genes (Rattus norvegicus)
Chrna2  (cholinergic receptor nicotinic alpha 2 subunit)


Additional Information