RGD Reference Report - Garlic Oil Suppressed Nitrosodiethylamine-Induced Hepatocarcinoma in Rats by Inhibiting PI3K-AKT-NF-κB Pathway. - Rat Genome Database

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Garlic Oil Suppressed Nitrosodiethylamine-Induced Hepatocarcinoma in Rats by Inhibiting PI3K-AKT-NF-κB Pathway.

Authors: Zhang, Cui-Li  Zeng, Tao  Zhao, Xiu-Lan  Xie, Ke-Qin 
Citation: Zhang CL, etal., Int J Biol Sci. 2015 Apr 25;11(6):643-51. doi: 10.7150/ijbs.10785. eCollection 2015.
RGD ID: 152177911
Pubmed: (View Article at PubMed) PMID:25999787
DOI: Full-text: DOI:10.7150/ijbs.10785

To explore the underlying mechanisms for the protective effects of garlic oil (GO) against nitrosodiethylamine (NDEA)-induced hepatocarcinoma, 60 male Wistar rats were randomized into 4 groups (n=15): control group, NDEA group, and two GO plus NDEA groups. The rats in GO plus NDEA groups were pretreated with GO (20 or 40 mg/kg) for 7 days. Then, all rats except those in control group were gavaged with NDEA for 20 weeks, and the rats in GO plus NDEA groups were continuously administered with GO. The results showed that GO co-treatment significantly suppressed the NDEA-induced increases of alpha fetal protein (AFP) level in serum, nuclear atypia in H&E staining, sirius red-positive areas and proliferating cell nuclear antigen (PCNA) expression. The molecular mechanisms exploration revealed that the protein levels of phosphatidylinositol 3 kinase (PI3K)-p85, PI3K-p110, total AKT, p-AKT (Ser473) and p-AKT (Thr308) in the liver of NDEA group rats were higher than those in control group rats. In addition, NDEA treatment induced IκB degradation and NF-κB p65 phosphorylation, and up-regulated the protein levels of downstream pro-inflammatory mediators. GO co-treatment significantly reversed all the above adverse effects induced by NDEA. These results suggested that the protective effects of GO against NDEA-induced hepatocarcinoma might be associated with the suppression of PI3K- AKT-NF-κB pathway.



Disease Annotations    

Gene-Chemical Interaction Annotations    
Objects Annotated

Genes (Rattus norvegicus)
Afp  (alpha-fetoprotein)
Akt1  (AKT serine/threonine kinase 1)
Nfkb1  (nuclear factor kappa B subunit 1)
Nos2  (nitric oxide synthase 2)
Pcna  (proliferating cell nuclear antigen)
Pik3ca  (phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha)
Pik3cb  (phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit beta)
Pik3r1  (phosphoinositide-3-kinase regulatory subunit 1)
Pik3r2  (phosphoinositide-3-kinase regulatory subunit 2)
Pik3r3  (phosphoinositide-3-kinase regulatory subunit 3)
Ptgs2  (prostaglandin-endoperoxide synthase 2)
Vegfa  (vascular endothelial growth factor A)

Genes (Mus musculus)
Afp  (alpha fetoprotein)
Akt1  (thymoma viral proto-oncogene 1)
Nfkb1  (nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105)
Nos2  (nitric oxide synthase 2, inducible)
Pcna  (proliferating cell nuclear antigen)
Pik3ca  (phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha)
Pik3cb  (phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta)
Pik3r1  (phosphoinositide-3-kinase regulatory subunit 1)
Pik3r2  (phosphoinositide-3-kinase regulatory subunit 2)
Pik3r3  (phosphoinositide-3-kinase regulatory subunit 3)
Ptgs2  (prostaglandin-endoperoxide synthase 2)
Vegfa  (vascular endothelial growth factor A)

Genes (Homo sapiens)
AFP  (alpha fetoprotein)
AKT1  (AKT serine/threonine kinase 1)
NFKB1  (nuclear factor kappa B subunit 1)
NOS2  (nitric oxide synthase 2)
PCNA  (proliferating cell nuclear antigen)
PIK3CA  (phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha)
PIK3CB  (phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta)
PIK3R1  (phosphoinositide-3-kinase regulatory subunit 1)
PIK3R2  (phosphoinositide-3-kinase regulatory subunit 2)
PIK3R3  (phosphoinositide-3-kinase regulatory subunit 3)
PTGS2  (prostaglandin-endoperoxide synthase 2)
VEGFA  (vascular endothelial growth factor A)

Objects referenced in this article
Gene RELA RELA proto-oncogene, NF-kB subunit Homo sapiens
Gene Rela v-rel reticuloendotheliosis viral oncogene homolog A (avian) Mus musculus
Gene Rela RELA proto-oncogene, NF-kB subunit Rattus norvegicus

Additional Information