RGD Reference Report - Rab38 and Rab32 control post-Golgi trafficking of melanogenic enzymes. - Rat Genome Database

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Rab38 and Rab32 control post-Golgi trafficking of melanogenic enzymes.

Authors: Wasmeier, Christina  Romao, Maryse  Plowright, Lynn  Bennett, Dorothy C  Raposo, Graça  Seabra, Miguel C 
Citation: Wasmeier C, etal., J Cell Biol. 2006 Oct 23;175(2):271-81. doi: 10.1083/jcb.200606050. Epub 2006 Oct 16.
RGD ID: 152025192
Pubmed: PMID:17043139   (View Abstract at PubMed)
PMCID: PMC2064568   (View Article at PubMed Central)
DOI: DOI:10.1083/jcb.200606050   (Journal Full-text)

A mutation in the small GTPase Rab38 gives rise to the mouse coat color phenotype "chocolate" (cht), implicating Rab38 in the regulation of melanogenesis. However, its role remains poorly characterized. We report that cht Rab38(G19V) is inactive and that the nearly normal pigmentation in cht melanocytes results from functional compensation by the closely related Rab32. In cht cells treated with Rab32-specific small interfering RNA, a dramatic loss of pigmentation is observed. In addition to mature melanosomes, Rab38 and Rab32 localize to perinuclear vesicles carrying tyrosinase and tyrosinase-related protein 1, consistent with a role in the intracellular sorting of these proteins. In Rab38/Rab32-deficient cells, tyrosinase appears to be mistargeted and degraded after exit from the trans-Golgi network (TGN). This suggests that Rab38 and Rab32 regulate a critical step in the trafficking of melanogenic enzymes, in particular, tyrosinase, from the TGN to melanosomes. This work identifies a key role for the Rab38/Rab32 subfamily of Rab proteins in the biogenesis of melanosomes and potentially other lysosome-related organelles.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Rab38Ratpositive regulation of melanin biosynthetic process  IMP  RGD 
Rab38Ratpositive regulation of protein localization to cell periphery  IMP  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Rab38Ratcell body  IDA MMO:0000686RGD 
Rab27aRatmelanosome  IDA MMO:0000686RGD 
Rab38Ratmelanosome located_inIDA MMO:0000686RGD 
Rab38Ratperinuclear region of cytoplasm  IDA MMO:0000686RGD 

Objects Annotated

Genes (Rattus norvegicus)
Rab27a  (RAB27A, member RAS oncogene family)
Rab38  (RAB38, member RAS oncogene family)


Additional Information