RGD Reference Report - Vesicular inhibitory amino acid transporter is a Cl-/gamma-aminobutyrate Co-transporter. - Rat Genome Database

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Vesicular inhibitory amino acid transporter is a Cl-/gamma-aminobutyrate Co-transporter.

Authors: Juge, Narinobu  Muroyama, Akiko  Hiasa, Miki  Omote, Hiroshi  Moriyama, Yoshinori 
Citation: Juge N, etal., J Biol Chem. 2009 Dec 11;284(50):35073-8. doi: 10.1074/jbc.M109.062414. Epub 2009 Oct 20.
RGD ID: 151665722
Pubmed: PMID:19843525   (View Abstract at PubMed)
PMCID: PMC2787368   (View Article at PubMed Central)
DOI: DOI:10.1074/jbc.M109.062414   (Journal Full-text)

The vesicular inhibitory amino acid transporter (VIAAT) is a synaptic vesicle protein responsible for the vesicular storage of gamma-aminobutyrate (GABA) and glycine which plays an essential role in GABAergic and glycinergic neurotransmission. The transport mechanism of VIAAT remains largely unknown. Here, we show that proteoliposomes containing purified VIAAT actively took up GABA upon formation of membrane potential (Deltapsi) (positive inside) but not DeltapH. VIAAT-mediated GABA uptake had an absolute requirement for Cl(-) and actually accompanied Cl(-) movement. Kinetic analysis indicated that one GABA molecule and two Cl(-) equivalents were transported during one transport cycle. VIAAT in which Glu(213) was specifically mutated to alanine completely lost the ability to take up both GABA and Cl(-). Essentially the same results were obtained with glycine, another substrate of VIAAT. These results demonstrated that VIAAT is a vesicular Cl(-) transporter that co-transports Cl(-) with GABA or glycine in a Deltapsi dependent manner. It is concluded that Cl(-) plays an essential role in vesicular storage of GABA and glycine.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc32a1Ratgamma-aminobutyric acid import involved_inIMP PMID:19843525UniProt 
Slc32a1Ratgamma-aminobutyric acid transport involved_inIMP PMID:19843525UniProt 
Slc32a1Ratglycine transport involved_inIMP PMID:19843525UniProt 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc32a1Ratgamma-aminobutyric acid transmembrane transporter activity enablesIMP PMID:19843525UniProt 
Slc32a1Ratglycine transmembrane transporter activity enablesIMP PMID:19843525UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Slc32a1  (solute carrier family 32 member 1)


Additional Information