RGD Reference Report - Targeting of CTCF to the nucleolus inhibits nucleolar transcription through a poly(ADP-ribosyl)ation-dependent mechanism. - Rat Genome Database

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Targeting of CTCF to the nucleolus inhibits nucleolar transcription through a poly(ADP-ribosyl)ation-dependent mechanism.

Authors: Torrano, Verónica  Navascués, Joaquín  Docquier, France  Zhang, Ru  Burke, Les J  Chernukhin, Igor  Farrar, Dawn  León, Javier  Berciano, María T  Renkawitz, Rainer  Klenova, Elena  Lafarga, Miguel  Delgado, M Dolores 
Citation: Torrano V, etal., J Cell Sci. 2006 May 1;119(Pt 9):1746-59. doi: 10.1242/jcs.02890. Epub 2006 Apr 4.
RGD ID: 151356507
Pubmed: PMID:16595548   (View Abstract at PubMed)
DOI: DOI:10.1242/jcs.02890   (Journal Full-text)

Multiple functions have been reported for the transcription factor and candidate tumour suppressor, CTCF. Among others, they include regulation of cell growth, differentiation and apoptosis, enhancer-blocking activity and control of imprinted genes. CTCF is usually localized in the nucleus and its subcellular distribution during the cell cycle is dynamic; CTCF was found associated with mitotic chromosomes and the midbody, suggesting different roles for CTCF at different stages of the cell cycle. Here we report the nucleolar localization of CTCF in several experimental model systems. Translocation of CTCF from nucleoplasm to the nucleolus was observed after differentiation of K562 myeloid cells and induction of apoptosis in MCF7 breast cancer cells. CTCF was also found in the nucleoli in terminally differentiated rat trigeminal ganglion neurons. Thus our data show that nucleolar localization of CTCF is associated with growth arrest. Interestingly, the 180 kDa poly(ADP-ribosyl)ated isoform of CTCF was predominantly found in the nucleoli fractions. By transfecting different CTCF deletion constructs into cell lines of different origin we demonstrate that the central zinc-finger domain of CTCF is the region responsible for nucleolar targeting. Analysis of subnucleolar localization of CTCF revealed that it is distributed homogeneously in both dense fibrillar and granular components of the nucleolus, but is not associated with fibrillar centres. RNA polymerase I transcription and protein synthesis were required to sustain nucleolar localization of CTCF. Notably, the labelling of active transcription sites by in situ run-on assays demonstrated that CTCF inhibits nucleolar transcription through a poly(ADP-ribosyl)ation-dependent mechanism.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CtcfRatdense fibrillar component  IDA  RGD 
CtcfRatgranular component  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ctcf  (CCCTC-binding factor)


Additional Information