RGD Reference Report - Pmch-deficiency in rats is associated with normal adipocyte differentiation and lower sympathetic adipose drive. - Rat Genome Database

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Pmch-deficiency in rats is associated with normal adipocyte differentiation and lower sympathetic adipose drive.

Authors: Mul, Joram D  O'Duibhir, Eoghan  Shrestha, Yogendra B  Koppen, Arjen  VargoviƧ, Peter  Toonen, Pim W  Zarebidaki, Eleen  Kvetnansky, Richard  Kalkhoven, Eric  Cuppen, Edwin  Bartness, Timothy J 
Citation: Mul JD, etal., PLoS One. 2013;8(3):e60214. doi: 10.1371/journal.pone.0060214. Epub 2013 Mar 26.
RGD ID: 150429944
Pubmed: PMID:23555928   (View Abstract at PubMed)
PMCID: PMC3608591   (View Article at PubMed Central)
DOI: DOI:10.1371/journal.pone.0060214   (Journal Full-text)

The orexigenic neuropeptide melanin-concentrating hormone (MCH), a product of Pmch, is an important mediator of energy homeostasis. Pmch-deficient rodents are lean and smaller, characterized by lower food intake, body-, and fat mass. Pmch is expressed in hypothalamic neurons that ultimately are components in the sympathetic nervous system (SNS) drive to white and interscapular brown adipose tissue (WAT, iBAT, respectively). MCH binds to MCH receptor 1 (MCH1R), which is present on adipocytes. Currently it is unknown if Pmch-ablation changes adipocyte differentiation or sympathetic adipose drive. Using Pmch-deficient and wild-type rats on a standard low-fat diet, we analyzed dorsal subcutaneous and perirenal WAT mass and adipocyte morphology (size and number) throughout development, and indices of sympathetic activation in WAT and iBAT during adulthood. Moreover, using an in vitro approach we investigated the ability of MCH to modulate 3T3-L1 adipocyte differentiation. Pmch-deficiency decreased dorsal subcutaneous and perirenal WAT mass by reducing adipocyte size, but not number. In line with this, in vitro 3T3-L1 adipocyte differentiation was unaffected by MCH. Finally, adult Pmch-deficient rats had lower norepinephrine turnover (an index of sympathetic adipose drive) in WAT and iBAT than wild-type rats. Collectively, our data indicate that MCH/MCH1R-pathway does not modify adipocyte differentiation, whereas Pmch-deficiency in laboratory rats lowers adiposity throughout development and sympathetic adipose drive during adulthood.



Phenotype Annotations    Click to see Annotation Detail View

Mammalian Phenotype

Objects Annotated

Genes (Rattus norvegicus)
Pmch  (pro-melanin-concentrating hormone)
Pmchm1Hubr  (pro-melanin-concentrating hormone; ENU induced mutant1, Hubr)

Strains
WI-Pmchm1Hubr  (NA)


Additional Information