RGD Reference Report - Phenotypic characterization of the Komeda miniature rat Ishikawa, an animal model of dwarfism caused by a mutation in Prkg2. - Rat Genome Database

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Phenotypic characterization of the Komeda miniature rat Ishikawa, an animal model of dwarfism caused by a mutation in Prkg2.

Authors: Tsuchida, Atsuko  Yokoi, Norihide  Namae, Misako  Fuse, Masanori  Masuyama, Taku  Sasaki, Masashi  Kawazu, Shoji  Komeda, Kajuro 
Citation: Tsuchida A, etal., Comp Med. 2008 Dec;58(6):560-7.
RGD ID: 150429793
Pubmed: PMID:19149413   (View Abstract at PubMed)
PMCID: PMC2710756   (View Article at PubMed Central)

The Komeda miniature rat Ishikawa (KMI) is a spontaneous animal model of dwarfism caused by a mutation in Prkg2, which encodes cGMP-dependent protein kinase type II (cGKII). This strain has been maintained as a segregating inbred strain for the mutated allele mri. In this study, we characterized the phenotype of the KMI strain, particularly growth traits, craniofacial measurements, and organ weights. The homozygous mutant (mri/mri) animals were approximately 70% to 80% of the size of normal, heterozygous (mri/+) animals in regard to body length, weight, and naso-occipital length of the calvarium, and the retroperitoneal fat of mri/mri rats was reduced greatly. In addition, among progeny of the (BNxKMI-mri/mri)F1xKMI-mri/mri backcross, animals with the KMI phenotype (mri/mri) were easily distinguished from those showing the wild-type phenotype (mri/+) by using growth traits such as body length and weight. Genetic analysis revealed that all of the backcrossed progeny exhibiting the KMI phenotype were homozygous for the KMI allele in the 1.2-cM region between D14Rat5 and D14Rat80 on chromosome 14, suggesting strongly that mri acts in a completely recessive manner. The KMI strain is the first and only rat model with a confirmed mutation in Prkg2 and is a valuable model for studying dwarfism and longitudinal growth traits in humans and for functional studies of cGKII.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
KMI/TkyRatDwarfism  IAGP compared to wild type and and heterozygous litter matesRGD 
PRKG2HumanDwarfism  ISOPrkg2 (Rattus norvegicus)DNA:deletion:cds (rat)RGD 
Prkg2RatDwarfism  IAGP DNA:deletion:cds (rat)RGD 
Prkg2MouseDwarfism  ISOPrkg2 (Rattus norvegicus)DNA:deletion:cds (rat)RGD 

Phenotype Annotations    Click to see Annotation Detail View

Mammalian Phenotype

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
KMI/TkyRatabnormal endochondral bone ossification  IAGP compared to wild type and and heterozygous litter matesRGD 
Prkg2Ratabnormal endochondral bone ossification  IAGP DNA:deletion:cds ratRGD 
KMI/TkyRatdecreased body length  IAGP compared to wild type and and heterozygous litter matesRGD 
Prkg2Ratdecreased body length  IAGP DNA:deletion:cds ratRGD 
KMI/TkyRatdecreased body weight  IAGP compared to wild type and and heterozygous litter matesRGD 
Prkg2Ratdecreased body weight  IAGP DNA:deletion:cds ratRGD 
KMI/TkyRatdecreased retroperitoneal fat pad weight  IAGP compared to wild type and and heterozygous litter matesRGD 
Prkg2Ratdecreased retroperitoneal fat pad weight  IAGP DNA:deletion:cds ratRGD 
KMI/TkyRatpostnatal growth retardation  IAGP compared to wild type and and heterozygous litter matesRGD 
Prkg2Ratpostnatal growth retardation  IAGP DNA:deletion:cds ratRGD 
KMI/TkyRatshort tail  IAGP compared to wild type and and heterozygous litter matesRGD 
Prkg2Ratshort tail  IAGP DNA:deletion:cds ratRGD 
KMI/TkyRatsmall neurocranium  IAGP compared to wild type and and heterozygous litter matesRGD 
Prkg2Ratsmall neurocranium  IAGP DNA:deletion:cds ratRGD 
Objects Annotated

Genes (Rattus norvegicus)
Prkg2  (protein kinase cGMP-dependent 2)

Genes (Mus musculus)
Prkg2  (protein kinase, cGMP-dependent, type II)

Genes (Homo sapiens)
PRKG2  (protein kinase cGMP-dependent 2)

Strains
KMI/Tky  (miniature rat ishikawa)


Additional Information