RGD Reference Report - Cyclic GMP-dependent protein kinase II is a molecular switch from proliferation to hypertrophic differentiation of chondrocytes. - Rat Genome Database

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Cyclic GMP-dependent protein kinase II is a molecular switch from proliferation to hypertrophic differentiation of chondrocytes.

Authors: Chikuda, Hirotaka  Kugimiya, Fumitaka  Hoshi, Kazuto  Ikeda, Toshiyuki  Ogasawara, Toru  Shimoaka, Takashi  Kawano, Hirotaka  Kamekura, Satoru  Tsuchida, Atsuko  Yokoi, Norihide  Nakamura, Kozo  Komeda, Kajuro  Chung, Ung-Il  Kawaguchi, Hiroshi 
Citation: Chikuda H, etal., Genes Dev. 2004 Oct 1;18(19):2418-29. doi: 10.1101/gad.1224204.
RGD ID: 150429792
Pubmed: PMID:15466490   (View Abstract at PubMed)
PMCID: PMC522991   (View Article at PubMed Central)
DOI: DOI:10.1101/gad.1224204   (Journal Full-text)

The Komeda miniature rat Ishikawa (KMI) is a naturally occurring mutant caused by an autosomal recessive mutation mri, which exhibits longitudinal growth retardation. Here we identified the mri mutation as a deletion in the rat gene encoding cGMP-dependent protein kinase type II (cGKII). KMIs showed an expanded growth plate and impaired bone healing with abnormal accumulation of postmitotic but nonhypertrophic chondrocytes. Ex vivo culture of KMI chondrocytes reproduced the differentiation impairment, which was restored by introducing the adenovirus-mediated cGKII gene. The expression of Sox9, an inhibitory regulator of hypertrophic differentiation, persisted in the nuclei of postmitotic chondrocytes of the KMI growth plate. Transfection experiments in culture systems revealed that cGKII attenuated the Sox9 functions to induce the chondrogenic differentiation and to inhibit the hypertrophic differentiation of chondrocytes. This attenuation of Sox9 was due to the cGKII inhibition of nuclear entry of Sox9. The impaired differentiation of cultured KMI chondrocytes was restored by the silencing of Sox9 through RNA interference. Hence, the present study for the first time shed light on a novel role of cGKII as a molecular switch, coupling the cessation of proliferation and the start of hypertrophic differentiation of chondrocytes through attenuation of Sox9 function.

Disease Annotations    
Dwarfism  (IAGP,ISO)

Gene Ontology Annotations    

Biological Process

Phenotype Annotations    

Mammalian Phenotype
Objects Annotated

Genes (Rattus norvegicus)
Prkg2  (protein kinase cGMP-dependent 2)

Genes (Mus musculus)
Prkg2  (protein kinase, cGMP-dependent, type II)

Genes (Homo sapiens)
PRKG2  (protein kinase cGMP-dependent 2)

KMI/Tky  (miniature rat ishikawa)

Additional Information