RGD Reference Report - Interaction of a Novel Chaperone PhLP2A With the Heat Shock Protein Hsp90. - Rat Genome Database

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Interaction of a Novel Chaperone PhLP2A With the Heat Shock Protein Hsp90.

Authors: Krzemień-Ojak, Łucja  Góral, Agnieszka  Joachimiak, Ewa  Filipek, Anna  Fabczak, Hanna 
Citation: Krzemień-Ojak Ł, etal., J Cell Biochem. 2017 Feb;118(2):420-429. doi: 10.1002/jcb.25669. Epub 2016 Oct 17.
RGD ID: 14695071
Pubmed: PMID:27496612   (View Abstract at PubMed)
DOI: DOI:10.1002/jcb.25669   (Journal Full-text)

PhLP2 is a small cytosolic protein that belongs to the highly conserved phosducin-like family of proteins. In amniote genomes there are two PhLP2 homologs, PhLP2A and PhLP2B. It has been shown that mammalian PhLP2A modulates the CCT/TRiC chaperonin activity during folding of cytoskeletal proteins. In order to better understand the function of PhLP2A in cellular protein quality control system, in the present study we have searched for its protein targets. Applying immunoprecipitation followed by mass spectrometry analysis we have identified Hsp90 as a partner of PhLP2A. With pull down experiments, we have confirmed this interaction in protein lysate and using purified proteins we have shown that PhLP2A interacts directly with Hsp90. Furthermore, the proximity ligation assay (PLA) performed on mIMCD-3 cells has shown that PhLP2A forms complexes with Hsp90 which are mainly localized in the cytoplasm of these cells. Further analysis has indicated that the level of PhLP2A increases after heat shock or radicicol treatment, similarly as the level of Hsp90, and that expression of PhLP2A after heat shock is regulated at the transcriptional level. Moreover, using recombinant luciferase we have shown that PhLP2A stabilizes this enzyme in a folding competent state and prevents its denaturation and aggregation. In addition, overexpression of PhLP2A in HEK-293 cells leads to increased heat stress resistance. Altogether, our results have shown that PhLP2A interacts with Hsp90 and exhibits molecular chaperone activity toward denatured proteins. J. Cell. Biochem. 118: 420-429, 2017. © 2016 Wiley Periodicals, Inc.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Pdcl3Ratcellular response to heat involved_inIDA PMID:27496612UniProt 
Pdcl3Ratprotein folding involved_inIDA PMID:27496612UniProt 

Molecular Function

  

Objects Annotated

Genes (Rattus norvegicus)
Hsp90aa1  (heat shock protein 90 alpha family class A member 1)
Hsp90ab1  (heat shock protein 90 alpha family class B member 1)
Pdcl3  (phosducin-like 3)

Objects referenced in this article
Gene Hsp90aa1-ps17 heat shock protein 90 alpha family class A member 1, pseudogene 17 Rattus norvegicus

Additional Information