RGD Reference Report - Establishment and phenotypic analysis of an Mstn knockout rat. - Rat Genome Database

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Establishment and phenotypic analysis of an Mstn knockout rat.

Authors: Gu, Hao  Cao, Yong  Qiu, Bin  Zhou, Zhiqiang  Deng, Ran  Chen, Zhuang  Li, Rongfeng  Li, Xueling  Wei, Qiang  Xia, Xianzhu  Yong, Weidong 
Citation: Gu H, etal., Biochem Biophys Res Commun. 2016 Aug 12;477(1):115-22. doi: 10.1016/j.bbrc.2016.06.030. Epub 2016 Jun 8.
RGD ID: 13831345
Pubmed: PMID:27289021   (View Abstract at PubMed)
DOI: DOI:10.1016/j.bbrc.2016.06.030   (Journal Full-text)

Myostatin (Mstn) is an inhibitor of myogenesis, regulating the number and size of skeletal myocytes. In addition to its myogenic regulatory function, Mstn plays important roles in the development of adipose tissues and in metabolism. In the present study, an Mstn knockout rat model was generated using the zinc finger nuclease (ZFN) technique in order to further investigate the function and mechanism of Mstn in metabolism. The knockout possesses a frame shift mutation resulting in an early termination codon and a truncated peptide of 109 amino acids rather than the full 376 amino acids. The absence of detectable mRNA confirmed successful knockout of Mstn. Relative to wild-type (WT) littermates, Knockout (KO) rats exhibited significantly greater body weight, body circumference, and muscle mass. However, no significant differences in grip force was observed, indicating that Mstn deletion results in greater muscle mass but not greater muscle fiber strength. Additionally, KO rats were found to possess less body fat relative to WT littermates, which is consistent with previous studies in mice and cattle. The aforementioned results indicate that Mstn knockout increases muscle mass while decreasing fat content, leading to observed increases in body weight and body circumference. The Mstn knockout rat model provides a novel means to study the role of Mstn in metabolism and Mstn-related muscle hypertrophy.



Phenotype Annotations    Click to see Annotation Detail View

Mammalian Phenotype

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MstnRatdecreased total body fat amount  IMP  RGD 
MstnRatincreased body weight sexual_dimorphism IMP compared to wild type maleRGD 
Mstnem1CqinRatincreased body weight sexual_dimorphism IMP compared to wild type maleRGD 
SD-Mstnem1CqinRatincreased body weight sexual_dimorphism IMP compared to wild type maleRGD 
MstnRatincreased muscle weight  IMP  RGD 
Objects Annotated

Genes (Rattus norvegicus)
Mstn  (myostatin)
Mstnem1Cqin  (myostatin; ZFN induced mutant 1, Cqin)

Strains
SD-Mstnem1Cqin  (NA)


Additional Information