RGD Reference Report - Enzymatic characteristics of an aldo-keto reductase family protein (AKR1C15) and its localization in rat tissues. - Rat Genome Database

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Enzymatic characteristics of an aldo-keto reductase family protein (AKR1C15) and its localization in rat tissues.

Authors: Endo, Satoshi  Matsunaga, Toshiyuki  Horie, Kenji  Tajima, Kazuo  Bunai, Yasuo  Carbone, Vincenzo  El-Kabbani, Ossama  Hara, Akira 
Citation: Endo S, etal., Arch Biochem Biophys. 2007 Sep 1;465(1):136-47. doi: 10.1016/j.abb.2007.05.008. Epub 2007 May 30.
RGD ID: 13792520
Pubmed: PMID:17574202   (View Abstract at PubMed)
DOI: DOI:10.1016/j.abb.2007.05.008   (Journal Full-text)

A member of the aldo-keto reductase superfamily, AKR1C15, was isolated via cDNA cloning, but its physiological function remains unknown. Here, we show that recombinant AKR1C15 is an NADPH-dependent reductase with broad substrate specificity for aromatic, alicyclic and aliphatic carbonyl compounds, including acetoin, 2,5-hexanedione, methylglyoxal, farnesal, retinals, 17-ketosteroids and monosaccharides. Especially, all-trans-retinal, alpha-diketones and lipid-derived aldehydes including 4-hydroxynonenal were excellent substrates showing low K(m) values (0.3-5.5 microM). Immunohistochemical and reverse transcription-PCR analyses revealed that AKR1C15 is highly expressed in rat bronchiolar Clara cells, type II alveolar cells, gastric parietal cells, the epithelial cells of the stomach and colon, and the brown adipocytes. The enzyme was not detected in cells of other rat tissues, but is consistently expressed in the vascular endothelial cells. These results suggest that AKR1C15 plays a role in retinoid, steroid, isoprenoid and carbohydrate metabolism, as well as a defense system, protecting against reactive carbonyl compounds.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Akr1c15Ratcytoplasm located_inIDA PMID:17574202UniProt 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Akr1c15Rataldo-keto reductase (NADPH) activity enablesIDA PMID:17574202UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Akr1c15  (aldo-keto reductase family 1, member C15)


Additional Information