RGD Reference Report - Synapsin II. Mapping of a domain in the NH2-terminal region which binds to small synaptic vesicles. - Rat Genome Database

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Synapsin II. Mapping of a domain in the NH2-terminal region which binds to small synaptic vesicles.

Authors: Thiel, G  Südhof, T C  Greengard, P 
Citation: Thiel G, etal., J Biol Chem. 1990 Sep 25;265(27):16527-33.
RGD ID: 13702354
Pubmed: PMID:2118908   (View Abstract at PubMed)

The synapsins are a family of neuron-specific phosphoproteins that selectively bind to small synaptic vesicles in the presynaptic nerve terminal. Using the cDNA encoding rat synapsin IIb, we employed an Escherichia coli expression system to synthesize a variety of fusion proteins containing a truncated protein A linked to different portions of the NH2-terminal region of synapsin II. The recombinant proteins were purified by IgG-Sepharose chromatography and tested in vitro for their ability to bind to purified synaptic vesicles. These experiments identified a region between amino acids 43 and 121 of the amino-terminal portion of synapsin II which binds to synaptic vesicles. Mild trypsinization of synaptic vesicles reduces binding of recombinant proteins to synaptic vesicles, suggesting that the interaction between synapsin II and the vesicles is in part mediated by a synaptic vesicle protein. The 42 NH2-terminal amino acids of synapsin II are not necessary for binding to synaptic vesicles, although this domain contains the phosphorylation site for cAMP-dependent protein kinase.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Syn2Ratextrinsic component of synaptic vesicle membrane is_active_inIDA PMID:2118908SynGO 
Syn2Ratextrinsic component of synaptic vesicle membrane is_active_inIEP PMID:2118908SynGO 
Syn2Ratextrinsic component of synaptic vesicle membrane is_active_inIMP PMID:2118908SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Syn2  (synapsin II)


Additional Information