RGD Reference Report - Molecular profiling of synaptic vesicle docking sites reveals novel proteins but few differences between glutamatergic and GABAergic synapses. - Rat Genome Database

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Molecular profiling of synaptic vesicle docking sites reveals novel proteins but few differences between glutamatergic and GABAergic synapses.

Authors: Boyken, Janina  Grønborg, Mads  Riedel, Dietmar  Urlaub, Henning  Jahn, Reinhard  Chua, John Jia En 
Citation: Boyken J, etal., Neuron. 2013 Apr 24;78(2):285-97. doi: 10.1016/j.neuron.2013.02.027.
RGD ID: 13702180
Pubmed: PMID:23622064   (View Abstract at PubMed)
DOI: DOI:10.1016/j.neuron.2013.02.027   (Journal Full-text)

Neurotransmission involves calcium-triggered fusion of docked synaptic vesicles at specialized presynaptic release sites. While many of the participating proteins have been identified, the molecular composition of these sites has not been characterized comprehensively. Here, we report a procedure to biochemically isolate fractions highly enriched in docked synaptic vesicles. The fraction is largely free of postsynaptic proteins and most other organelles while containing most known synaptic vesicle and active zone proteins. Numerous presynaptic transmembrane proteins were also identified, together with over 30 uncharacterized proteins, many of which are evolutionarily conserved. Quantitative proteomic comparison of glutamate- and GABA-specific docking complexes revealed that, except of neurotransmitter-specific enzymes and transporters, only few proteins were selectively enriched in either fraction. We conclude that the core machinery involved in vesicle docking and exocytosis does not show compositional differences between the two types of synapses.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Phb1Ratextrinsic component of presynaptic active zone membrane is_active_inEXP PMID:23622064SynGO 
Phb1Ratextrinsic component of presynaptic active zone membrane is_active_inIDA PMID:23622064SynGO 
Atp6v1b1Ratextrinsic component of synaptic vesicle membrane is_active_inEXP PMID:23622064SynGO 
Atp6v1b1Ratextrinsic component of synaptic vesicle membrane is_active_inIEP PMID:23622064SynGO 
Atp6v1b1Ratextrinsic component of synaptic vesicle membrane is_active_inIDA PMID:23622064SynGO 
Atp6v1hRatextrinsic component of synaptic vesicle membrane is_active_inEXP PMID:23622064SynGO 
Atp6v1hRatextrinsic component of synaptic vesicle membrane is_active_inIEP PMID:23622064SynGO 
Atp6v1hRatextrinsic component of synaptic vesicle membrane is_active_inIDA PMID:23622064SynGO 
Syn1Ratextrinsic component of synaptic vesicle membrane is_active_inEXP PMID:23622064SynGO 
Syn1Ratextrinsic component of synaptic vesicle membrane is_active_inIDA PMID:23622064SynGO 
Syn2Ratextrinsic component of synaptic vesicle membrane is_active_inEXP PMID:23622064SynGO 
Syn2Ratextrinsic component of synaptic vesicle membrane is_active_inIDA PMID:23622064SynGO 
Syn3Ratextrinsic component of synaptic vesicle membrane is_active_inEXP PMID:23622064SynGO 
Syn3Ratextrinsic component of synaptic vesicle membrane is_active_inIDA PMID:23622064SynGO 
Atp2b1RatGABA-ergic synapse is_active_inIDA PMID:23622064SynGO 
Atp2b1RatGABA-ergic synapse is_active_inEXP PMID:23622064SynGO 
Atp2b2RatGABA-ergic synapse is_active_inEXP PMID:23622064SynGO 
Atp2b2RatGABA-ergic synapse is_active_inIDA PMID:23622064SynGO 
C1qbpRatGABA-ergic synapse is_active_inEXP PMID:23622064SynGO 
C1qbpRatGABA-ergic synapse is_active_inIDA PMID:23622064SynGO 
Flot1RatGABA-ergic synapse is_active_inIDA PMID:23622064SynGO 
Flot1RatGABA-ergic synapse is_active_inEXP PMID:23622064SynGO 
NptnRatGABA-ergic synapse is_active_inIDA PMID:23622064SynGO 
NptnRatGABA-ergic synapse is_active_inEXP PMID:23622064SynGO 
Phb1RatGABA-ergic synapse is_active_inEXP PMID:23622064SynGO 
Phb1RatGABA-ergic synapse is_active_inIDA PMID:23622064SynGO 
Phb2RatGABA-ergic synapse is_active_inIDA PMID:23622064SynGO 
Phb2RatGABA-ergic synapse is_active_inEXP PMID:23622064SynGO 
Rps27RatGABA-ergic synapse is_active_inIDA PMID:23622064SynGO 
Rps27RatGABA-ergic synapse is_active_inEXP PMID:23622064SynGO 
Atp2b1Ratglutamatergic synapse is_active_inIDA PMID:23622064SynGO 
Atp2b1Ratglutamatergic synapse is_active_inEXP PMID:23622064SynGO 
Atp2b2Ratglutamatergic synapse is_active_inEXP PMID:23622064SynGO 
Atp2b2Ratglutamatergic synapse is_active_inIDA PMID:23622064SynGO 
Atp2b4Ratglutamatergic synapse is_active_inIDA PMID:23622064SynGO 
Atp2b4Ratglutamatergic synapse is_active_inEXP PMID:23622064SynGO 
C1qbpRatglutamatergic synapse is_active_inEXP PMID:23622064SynGO 
C1qbpRatglutamatergic synapse is_active_inIDA PMID:23622064SynGO 
CanxRatglutamatergic synapse is_active_inEXP PMID:23622064SynGO 
CanxRatglutamatergic synapse is_active_inIDA PMID:23622064SynGO 
Flot1Ratglutamatergic synapse is_active_inIDA PMID:23622064SynGO 
Flot1Ratglutamatergic synapse is_active_inEXP PMID:23622064SynGO 
Flot2Ratglutamatergic synapse is_active_inIDA PMID:23622064SynGO 
Flot2Ratglutamatergic synapse is_active_inEXP PMID:23622064SynGO 
Gpm6aRatglutamatergic synapse is_active_inIDA PMID:23622064SynGO 
Gpm6aRatglutamatergic synapse is_active_inEXP PMID:23622064SynGO 
NptnRatglutamatergic synapse is_active_inIDA PMID:23622064SynGO 
NptnRatglutamatergic synapse is_active_inEXP PMID:23622064SynGO 
Phb1Ratglutamatergic synapse is_active_inEXP PMID:23622064SynGO 
Phb1Ratglutamatergic synapse is_active_inIDA PMID:23622064SynGO 
Phb2Ratglutamatergic synapse is_active_inIDA PMID:23622064SynGO 
Phb2Ratglutamatergic synapse is_active_inEXP PMID:23622064SynGO 
Rps27Ratglutamatergic synapse is_active_inIDA PMID:23622064SynGO 
Rps27Ratglutamatergic synapse is_active_inEXP PMID:23622064SynGO 
KalrnRatpresynapse is_active_inEXP PMID:23622064SynGO 
KalrnRatpresynapse is_active_inIDA PMID:23622064SynGO 
Ppfibp2Ratpresynapse is_active_inEXP PMID:23622064SynGO 
Ppfibp2Ratpresynapse is_active_inIDA PMID:23622064SynGO 
Rab1aRatpresynapse is_active_inEXP PMID:23622064SynGO 
Rab1aRatpresynapse is_active_inIDA PMID:23622064SynGO 
RogdiRatpresynapse is_active_inEXP PMID:23622064SynGO 
RogdiRatpresynapse is_active_inIDA PMID:23622064SynGO 
Rps27Ratpresynapse is_active_inEXP PMID:23622064SynGO 
Rps27Ratpresynapse is_active_inIDA PMID:23622064SynGO 
C1qbpRatpresynaptic active zone is_active_inEXP PMID:23622064SynGO 
C1qbpRatpresynaptic active zone is_active_inIDA PMID:23622064SynGO 
Flot1Ratpresynaptic active zone is_active_inEXP PMID:23622064SynGO 
Flot1Ratpresynaptic active zone is_active_inIDA PMID:23622064SynGO 
Phb2Ratpresynaptic active zone is_active_inEXP PMID:23622064SynGO 
Phb2Ratpresynaptic active zone is_active_inIDA PMID:23622064SynGO 
Atp2b1Ratpresynaptic active zone membrane is_active_inEXP PMID:23622064SynGO 
Atp2b1Ratpresynaptic active zone membrane is_active_inIDA PMID:23622064SynGO 
Atp2b2Ratpresynaptic active zone membrane is_active_inEXP PMID:23622064SynGO 
Atp2b2Ratpresynaptic active zone membrane is_active_inIDA PMID:23622064SynGO 
Atp2b4Ratpresynaptic active zone membrane is_active_inEXP PMID:23622064SynGO 
Atp2b4Ratpresynaptic active zone membrane is_active_inIDA PMID:23622064SynGO 
CanxRatpresynaptic active zone membrane is_active_inEXP PMID:23622064SynGO 
CanxRatpresynaptic active zone membrane is_active_inIDA PMID:23622064SynGO 
Flot2Ratpresynaptic active zone membrane is_active_inEXP PMID:23622064SynGO 
Flot2Ratpresynaptic active zone membrane is_active_inIDA PMID:23622064SynGO 
Gpm6aRatpresynaptic active zone membrane is_active_inEXP PMID:23622064SynGO 
Gpm6aRatpresynaptic active zone membrane is_active_inIDA PMID:23622064SynGO 
NptnRatpresynaptic active zone membrane is_active_inEXP PMID:23622064SynGO 
NptnRatpresynaptic active zone membrane is_active_inIDA PMID:23622064SynGO 
Atg9aRatsynaptic vesicle is_active_inEXP PMID:23622064SynGO 
Atg9aRatsynaptic vesicle is_active_inIDA PMID:23622064SynGO 
Vps45Ratsynaptic vesicle is_active_inIDA PMID:23622064SynGO 
Atp6v0a4Ratsynaptic vesicle membrane is_active_inEXP PMID:23622064SynGO 
Atp6v0a4Ratsynaptic vesicle membrane is_active_inIEP PMID:23622064SynGO 
Atp6v0a4Ratsynaptic vesicle membrane is_active_inIDA PMID:23622064SynGO 
Atp8a1Ratsynaptic vesicle membrane is_active_inEXP PMID:23622064SynGO 
Atp8a1Ratsynaptic vesicle membrane is_active_inIDA PMID:23622064SynGO 
Ptprn2Ratsynaptic vesicle membrane is_active_inEXP PMID:23622064SynGO 
Ptprn2Ratsynaptic vesicle membrane is_active_inIDA PMID:23622064SynGO 
Stx1aRatsynaptic vesicle membrane is_active_inIDA PMID:23622064SynGO 
Sv2aRatsynaptic vesicle membrane is_active_inEXP PMID:23622064SynGO 
Sv2aRatsynaptic vesicle membrane is_active_inIDA PMID:23622064SynGO 
Sv2bRatsynaptic vesicle membrane is_active_inEXP PMID:23622064SynGO 
Sv2bRatsynaptic vesicle membrane is_active_inIDA PMID:23622064SynGO 
Sv2cRatsynaptic vesicle membrane is_active_inEXP PMID:23622064SynGO 
Sv2cRatsynaptic vesicle membrane is_active_inIDA PMID:23622064SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Atg9a  (autophagy related 9A)
Atp2b1  (ATPase plasma membrane Ca2+ transporting 1)
Atp2b2  (ATPase plasma membrane Ca2+ transporting 2)
Atp2b4  (ATPase plasma membrane Ca2+ transporting 4)
Atp6v0a4  (ATPase H+ transporting V0 subunit a4)
Atp6v1b1  (ATPase H+ transporting V1 subunit B1)
Atp6v1h  (ATPase H+ transporting V1 subunit H)
Atp8a1  (ATPase phospholipid transporting 8A1)
C1qbp  (complement C1q binding protein)
Canx  (calnexin)
Flot1  (flotillin 1)
Flot2  (flotillin 2)
Gpm6a  (glycoprotein m6a)
Kalrn  (kalirin, RhoGEF kinase)
Nptn  (neuroplastin)
Phb1  (prohibitin 1)
Phb2  (prohibitin 2)
Ppfibp2  (PPFIA binding protein 2)
Ptprn2  (protein tyrosine phosphatase, receptor type N2)
Rab1a  (RAB1A, member RAS oncogene family)
Rogdi  (rogdi atypical leucine zipper)
Rps27  (ribosomal protein S27)
Stx1a  (syntaxin 1A)
Sv2a  (synaptic vesicle glycoprotein 2a)
Sv2b  (synaptic vesicle glycoprotein 2b)
Sv2c  (synaptic vesicle glycoprotein 2c)
Syn1  (synapsin I)
Syn2  (synapsin II)
Syn3  (synapsin III)
Vps45  (vacuolar protein sorting 45 homolog)

Objects referenced in this article
Gene AABR07014974.1 null Rattus norvegicus

Additional Information