RGD Reference Report - Activities of hepatic cytosolic and mitochondrial forms of serine hydroxymethyltransferase and hepatic glycine concentration are affected by vitamin B-6 intake in rats. - Rat Genome Database

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Activities of hepatic cytosolic and mitochondrial forms of serine hydroxymethyltransferase and hepatic glycine concentration are affected by vitamin B-6 intake in rats.

Authors: Scheer, JB  Mackey, AD  Gregory JF, 3RD 
Citation: Scheer JB, etal., J Nutr 2005 Feb;135(2):233-8.
RGD ID: 1359815
Pubmed: PMID:15671219   (View Abstract at PubMed)

Serine hydroxymethyltransferase (SHMT) is a pyridoxal phosphate (PLP)-dependent enzyme that exists as cytosolic and mitochondrial isozymes that catalyze the reversible interconversion of serine and tetrahydrofolate (THF) to glycine and 5,10-methyleneTHF. SHMT is a major source of one-carbon units for cellular metabolism, but its sensitivity to various degrees of altered vitamin B-6 nutritional status has not been determined. In this study, cytosolic and mitochondrial SHMT activities were measured in liver from rats fed dietary pyridoxine (PN) ranging from adequate to deficient levels (2, 1, 0.5, 0.1, and 0 mg PN/kg diet; n = 10 per group). Both mitochondrial and cytosolic SHMT activities increased (P < 0.001) with increasing dietary PN over this range, and activities were a linear function of liver PLP concentration. Mitochondrial SHMT comprised approximately 70% of total activity. Assays conducted with and without in vitro addition of PLP indicated that total SHMT (apo- and holoenzyme forms) varied with dietary PN for each isoform, but that the proportion of each present as the apoenzyme was not affected by PN intake. This aspect of SHMT nutritional regulation differs from that of many other PLP-dependent enzymes. Hepatic glycine concentration was inversely related to vitamin B-6 intake (P < 0.05), which suggests a functional effect of altered SHMT activity. Overall these results demonstrate the potential for disruption of SHMT-mediated one-carbon metabolism by inadequate vitamin B-6 intake.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Shmt1Ratglycine metabolic process  TAS  RGD 
Shmt1Ratglycine metabolic process  IDA  RGD 
Shmt2Ratglycine metabolic process  IDA  RGD 
Shmt2Ratglycine metabolic process  TAS  RGD 
Shmt1RatL-serine metabolic process  TAS  RGD 
Shmt2RatL-serine metabolic process  TAS  RGD 

Molecular Function

  

Molecular Pathway Annotations    Click to see Annotation Detail View
Objects Annotated

Genes (Rattus norvegicus)
Shmt1  (serine hydroxymethyltransferase 1)
Shmt2  (serine hydroxymethyltransferase 2)

Genes (Homo sapiens)
SHMT1  (serine hydroxymethyltransferase 1)
SHMT2  (serine hydroxymethyltransferase 2)


Additional Information