RGD Reference Report - Cloning of an intracellular protein that binds selectively to mitogenic acidic fibroblast growth factor. - Rat Genome Database

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Cloning of an intracellular protein that binds selectively to mitogenic acidic fibroblast growth factor.

Authors: Kolpakova, E  Wiedlocha, A  Stenmark, H  Klingenberg, O  Falnes, PO  Olsnes, S 
Citation: Kolpakova E, etal., Biochem J 1998 Nov 15;336 ( Pt 1):213-22.
RGD ID: 1359054
Pubmed: PMID:9806903   (View Abstract at PubMed)
PMCID: PMC1219860   (View Article at PubMed Central)

In addition to its extracellular action, there is evidence that acidic fibroblast growth factor (aFGF) acts inside cells. To identify intracellular proteins interacting with aFGF, we screened a HeLa cell library in the yeast two-hybrid system using pLex-aFGF as a bait. A clone binding to aFGF, but not to the non-mitogenic mutant aFGF-K132E, was isolated and characterized. The insert contained an open reading frame corresponding to a novel protein of 42 kDa. The protein, termed aFGF intracellular binding protein (FIBP), is mainly hydrophilic and does not contain an N-terminal signal sequence. In vitro-translated FIBP bound specifically to a fusion protein of maltose-binding protein and aFGF. FIBP became post-translationally associated with microsomes added to the cell-free protein synthesizing system, and the membrane-associated protein bound aFGF with high efficiency. Immunoblots and fluorescence microscopy demonstrated that the protein is present in nuclei and, to a lesser extent, associated with mitochondria and other cytoplasmic membranes. The possibility is discussed that FIBP may be involved in the mitogenic action of aFGF.



Objects referenced in this article
Gene Fibp FGF1 intracellular binding protein Rattus norvegicus

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