RGD Reference Report - Linkage and microarray analyses of susceptibility genes in ACI/Seg rats: a model for prostate cancers in the aged. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources
Advanced Search (OLGA)

Linkage and microarray analyses of susceptibility genes in ACI/Seg rats: a model for prostate cancers in the aged.

Authors: Yamashita, S  Suzuki, S  Nomoto, T  Kondo, Y  Wakazono, K  Tsujino, Y  Sugimura, T  Shirai, T  Homma, Y  Ushijima, T 
Citation: Yamashita S, etal., Cancer Res 2005 Apr 1;65(7):2610-6.
RGD ID: 1358944
Pubmed: PMID:15805257   (View Abstract at PubMed)
DOI: DOI:10.1158/0008-5472.CAN-04-2932   (Journal Full-text)

ACI/Seg (ACI) rats develop prostate cancers spontaneously with aging, similar to humans. Here, to identify genes involved in prostate cancer susceptibility, we did linkage analysis and oligonucleotide microarray analysis. Linkage analysis was done using 118 effective rats, and prostate cancer susceptibility 1 (Pcs1), whose ACI allele dominantly induced prostate cancers, was mapped on chromosome 19 [logarithm of odds (LOD) score of 5.0]. PC resistance 1 (Pcr1), whose ACI allele dominantly and paradoxically suppressed the size of prostate cancers, was mapped on chromosome 2 (LOD score of 5.0). When linkage analysis was done in 51 rats with single or no macroscopic testicular tumors, which had larger prostates and higher testosterone levels than those with bilateral testicular tumors, Pcs2 and Pcr2 were mapped on chromosomes 20 and 1, respectively. By oligonucleotide microarray analysis with 8,800 probe sets and confirmation by quantitative reverse transcription-PCR, only two genes within these four loci were found to be differentially expressed >1.8-fold. Membrane metalloendopeptidase (Mme), known to inhibit androgen-independent growth of prostate cancers, on Pcr1 was expressed 2.0- to 5.5-fold higher in the ACI prostate, in accordance with its paradoxical effect. Cdkn1a on Pcs2 was expressed 1.5- to 4.5-fold lower in the ACI prostate. Additionally, genes responsible for testicular tumors and unilateral renal agenesis were mapped on chromosomes 11 and 14, respectively. These results showed that prostate cancer susceptibility of ACI rats involves at least four loci, and suggested Mme and Cdkn1a as candidates for Pcr1 and Pcs2.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Prcr1RatProstatic Neoplasms  IAGP  RGD 
Prcr2RatProstatic Neoplasms  IAGP  RGD 
Prcs1RatProstatic Neoplasms  IAGP  RGD 
Prcs2RatProstatic Neoplasms  IAGP  RGD 
Tescar1RatTesticular Neoplasms  IAGP  RGD 

Phenotype Annotations    Click to see Annotation Detail View

Related Phenotype Data for this Reference

Phenominer Options:  View all phenotype data for this reference  |  Download all phenotype data for this reference

Select a value below to view phenotype data for a specific strain, measurment, or condition.

Rat Strains:
Clinical Measurements:
Experimental Conditions:
Measurement Methods:
Objects Annotated

QTLs
Prcr1  (Prostate cancer resistance QTL 1)
Prcr2  (Prostate cancer resistance QTL 2)
Prcs1  (Prostate cancer susceptibility QTL 1)
Prcs2  (Prostate cancer susceptibility QTL 2)
Tescar1  (Testicular tumor resistance QTL 1)

Strains
ACI/SegHsd  (NA)
F344/Jcl  (NA)


Additional Information