RGD Reference Report - A quantitative trait locus for aortic smooth muscle cell number acting independently of blood pressure: implicating the angiotensin receptor AT1B gene as a candidate. - Rat Genome Database

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A quantitative trait locus for aortic smooth muscle cell number acting independently of blood pressure: implicating the angiotensin receptor AT1B gene as a candidate.

Authors: Dutil, J  Eliopoulos, V  Marchand, EL  Devlin, AM  Tremblay, J  Prithiviraj, K  Hamet, P  Migneault, A  DeBlois, D  Deng, AY 
Citation: Dutil J, etal., Physiol Genomics 2005 Mar 1;.
RGD ID: 1357419
Pubmed: PMID:15741507   (View Abstract at PubMed)
DOI: DOI:10.1152/physiolgenomics.00063.2004   (Journal Full-text)

Vascular hyperplasia may be involved in the remodeling of vasculature. It was unknown if there were genetic determinants for aortic smooth muscle cell number (SMCN) and, if yes, whether they acted independently of those for blood pressure (BP). To unravel this issue, we utilized congenic strains previously constructed for BP studies. These strains were made by replacing various Chromosome 2 segments of the Dahl salt-sensitive (S) rat with those of the Milan normotensive rat (MNS). We measured and compared SMCN in aortic cross sectional areas and BPs of these strains. Consequently, a quantitative trait locus (QTL) for SMCN was localized to a chromosome region not containing a BP QTL, but harboring the locus for the angiotensin II receptor AT1B (Agtr1b). Agtr1b became a candidate for the SMCN QTL because a) two significant mutations were found in the coding region between S and all congenic strains possessing the MNS alleles and b) contractile responses to angiotensin II were significantly and selectively reduced in congenic rats harboring the MNS alleles of the SMCN QTL as compared to S rats. The current investigation presents the first line of evidence that a QTL for aortic SMCN exists, and it acts independently of QTLs for BP. The relevant congenic strains developed therein potentially provide novel mammalian models for the studies of vascular remodeling disorders.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Smcn1Rataortic disease  IAGP  RGD 

Phenotype Annotations    Click to see Annotation Detail View

Mammalian Phenotype

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Smcn1Ratvascular smooth muscle hyperplasia  IAGP  RGD 

Objects Annotated

QTLs
Smcn1  (Smooth muscle cell number QTL 1)

Objects referenced in this article
Gene Agtr1b angiotensin II receptor, type 1b Rattus norvegicus
Strain SS.MNS-(D2Mit6-Adh1)/Ayd null Rattus norvegicus
Strain SS.MNS-(D2Mit6-D2Rat166)/Ayd null Rattus norvegicus
Strain SS.MNS-(Mme-D2Rat131)/Ayd null Rattus norvegicus

Additional Information