RGD Reference Report - TAP/NXF1, the primary mRNA export receptor, specifically interacts with a neuronal RNA-binding protein HuD. - Rat Genome Database

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TAP/NXF1, the primary mRNA export receptor, specifically interacts with a neuronal RNA-binding protein HuD.

Authors: Saito, K  Fujiwara, T  Katahira, J  Inoue, K  Sakamoto, H 
Citation: Saito K, etal., Biochem Biophys Res Commun 2004 Aug 20;321(2):291-7.
RGD ID: 1357206
Pubmed: PMID:15358174   (View Abstract at PubMed)
DOI: DOI:10.1016/j.bbrc.2004.06.140   (Journal Full-text)

Hu proteins are RNA-binding proteins that are implicated in the control of stabilization, nuclear export, and/or translation of specific mRNAs with AU-rich elements (AREs) in the 3'-untranslated region. Three neuron-specific Hu proteins (HuD, HuB, and HuC), but not a ubiquitously expressed Hu protein HuR, have an activity to induce neurite outgrowth when they are overexpressed in a rat neuronal cell line PC12. Here we show that TAP/NXF1, the primary export receptor for the bulk mRNA, is a specific binding partner for HuD. In vitro binding experiments using recombinant proteins revealed that the interaction between TAP and HuD is direct and that HuD can form a ternary complex together with both TAP and RNA. Interestingly, HuR does not interact with TAP. These results suggest that HuD acts as a novel adaptor protein to recruit TAP for efficient export of ARE-containing mRNAs in neuronal cells.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Nxf1Ratprotein binding  IPIRGD:1557144 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Nxf1  (nuclear RNA export factor 1)


Additional Information