RGD Reference Report - Exogenous gene transfer of Rab38 small GTPase ameliorates aberrant lung surfactant homeostasis in Ruby rats. - Rat Genome Database

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Exogenous gene transfer of Rab38 small GTPase ameliorates aberrant lung surfactant homeostasis in Ruby rats.

Authors: Osanai, Kazuhiro  Nakase, Keisuke  Sakuma, Takashi  Nishiki, Kazuaki  Nojiri, Masafumi  Kato, Ryo  Saito, Masatoshi  Fujimoto, Yuki  Mizuno, Shiro  Toga, Hirohisa 
Citation: Osanai K, etal., Respir Res. 2017 Apr 24;18(1):70. doi: 10.1186/s12931-017-0549-2.
RGD ID: 13524861
Pubmed: PMID:28438206   (View Abstract at PubMed)
PMCID: PMC5402648   (View Article at PubMed Central)
DOI: DOI:10.1186/s12931-017-0549-2   (Journal Full-text)


BACKGROUND: Rab38 small GTPase regulates intracellular transport in melanocytes and alveolar type II epithelial cells. Ruby rats carrying Rab38 and other gene mutations exhibit oculocutaneous albinism, bleeding diathesis, and hence, are a rat model of human Hermansky-Pudlak syndrome (HPS). We previously showed that Long Evans Cinnamon (LEC) rats, one strain of the Ruby rats, developed aberrant lung surfactant homeostasis with remarkably enlarged lamellar bodies in alveolar type II cells.
METHODS: A replication-deficient recombinant adenovirus expressing rat Rab38 (Ad-Rab38) was constructed. Alveolar type II cells were isolated from the LEC rats and tested for lung surfactant phosphatidylcholine secretion. The rats were also examined whether exogenous expression of Ad- Rab38 could rescue the altered lung surfactant homeostasis in the lungs.
RESULTS: Isolated type II cells infected with Ad-Rab38 exhibited improved secretion patterns of [3H]phosphatidylcholine, i.e. increased basal hyposecretion and decreased agonist-induced hypersecretion. Endobronchial administration of Ad-Rab38 improved the morphology of type II cells and lamellar bodies, reducing their sizes close to those of wild-type rats. The increased amounts of phosphatidylcholine and surfactant protein B in the lamellar body fractions were decreased in the Ad-Rab38 infected lungs.
CONCLUSIONS: These results provide strong evidence that the aberrant lung surfactant homeostasis in the LEC rats is caused by Rab38 deficit, and suggest that endobronchial delivery of the responsive transgene could be an effective method to ameliorate the abnormal lung phenotype in the animal model of HPS.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Rab38Ratpositive regulation of phosphatidylcholine biosynthetic process  IDA  RGD 

Phenotype Annotations    Click to see Annotation Detail View

Mammalian Phenotype

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
LEC/CrljRatabnormal surfactant secretion  IAGP  RGD 
LEC/CrljRatenlarged alveolar lamellar bodies  IAGP  RGD 
Rab38Ratenlarged alveolar lamellar bodies  IMP  RGD 
Rab38ruRatenlarged alveolar lamellar bodies  IMP  RGD 
Objects Annotated

Genes (Rattus norvegicus)
Rab38  (RAB38, member RAS oncogene family)
Rab38ru  (Rab38, member of RAS oncogene family, ruby allele)

Strains
LEC/Crlj  (Long Evans Cinnamon)


Additional Information