RGD Reference Report - Surfactant secretion in LRRK2 knock-out rats: changes in lamellar body morphology and rate of exocytosis. - Rat Genome Database

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Surfactant secretion in LRRK2 knock-out rats: changes in lamellar body morphology and rate of exocytosis.

Authors: Miklavc, Pika  Ehinger, Konstantin  Thompson, Kristin E  Hobi, Nina  Shimshek, Derya R  Frick, Manfred 
Citation: Miklavc P, etal., PLoS One. 2014 Jan 21;9(1):e84926. doi: 10.1371/journal.pone.0084926. eCollection 2014.
RGD ID: 13462051
Pubmed: PMID:24465451   (View Abstract at PubMed)
PMCID: PMC3897396   (View Article at PubMed Central)
DOI: DOI:10.1371/journal.pone.0084926   (Journal Full-text)

Leucine-rich repeat kinase 2 (LRRK2) is known to play a role in the pathogenesis of various diseases including Parkinson disease, morbus Crohn, leprosy and cancer. LRRK2 is suggested to be involved in a number of cell biological processes such as vesicular trafficking, transcription, autophagy and lysosomal pathways. Recent histological studies of lungs of LRRK2 knock-out (LRRK2 -/-) mice revealed significantly enlarged lamellar bodies (LBs) in alveolar type II (ATII) epithelial cells. LBs are large, lysosome-related storage organelles for pulmonary surfactant, which is released into the alveolar lumen upon LB exocytosis. In this study we used high-resolution, subcellular live-cell imaging assays to investigate whether similar morphological changes can be observed in primary ATII cells from LRRK2 -/- rats and whether such changes result in altered LB exocytosis. Similarly to the report in mice, ATII cells from LRRK2 -/- rats contained significantly enlarged LBs resulting in a >50% increase in LB volume. Stimulation of ATII cells with ATP elicited LB exocytosis in a significantly increased proportion of cells from LRRK2 -/- animals. LRRK2 -/- cells also displayed increased intracellular Ca(2+) release upon ATP treatment and significant triggering of LB exocytosis. These findings are in line with the strong Ca(2+)-dependence of LB fusion activity and suggest that LRRK2 -/- affects exocytic response in ATII cells via modulating intracellular Ca(2+) signaling. Post-fusion regulation of surfactant secretion was unaltered. Actin coating of fused vesicles and subsequent vesicle compression to promote surfactant expulsion were comparable in cells from LRRK2 -/- and wt animals. Surprisingly, surfactant (phospholipid) release from LRRK2 -/- cells was reduced following stimulation of LB exocytosis possibly due to impaired LB maturation and surfactant loading of LBs. In summary our results suggest that LRRK2 -/- affects LB size, modulates intracellular Ca(2+) signaling and promotes LB exocytosis upon stimulation of ATII cells with ATP.



Phenotype Annotations    

Mammalian Phenotype
Objects Annotated

Genes (Rattus norvegicus)
Lrrk2  (leucine-rich repeat kinase 2)
Lrrk2em1Sage  (leucine-rich repeat kinase 2; zinc finger nuclease induced mutant 1, Sigma Advanced Genetic Engineering Labs)

Strains
LE-Lrrk2em1Sage-/-  (NA)


Additional Information