RGD Reference Report - Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis. - Rat Genome Database

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Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis.

Authors: Pavlos, Nathan J  Grønborg, Mads  Riedel, Dietmar  Chua, John J E  Boyken, Janina  Kloepper, Tobias H  Urlaub, Henning  Rizzoli, Silvio O  Jahn, Reinhard 
Citation: Pavlos NJ, etal., J Neurosci. 2010 Oct 6;30(40):13441-53. doi: 10.1523/JNEUROSCI.0907-10.2010.
RGD ID: 13432355
Pubmed: PMID:20926670   (View Abstract at PubMed)
PMCID: PMC6634719   (View Article at PubMed Central)
DOI: DOI:10.1523/JNEUROSCI.0907-10.2010   (Journal Full-text)

Rab GTPases are molecular switches that orchestrate protein complexes before membrane fusion reactions. In synapses, Rab3 and Rab5 proteins have been implicated in the exo-endocytic cycling of synaptic vesicles (SVs), but an involvement of additional Rabs cannot be excluded. Here, combining high-resolution mass spectrometry and chemical labeling (iTRAQ) together with quantitative immunoblotting and fluorescence microscopy, we have determined the exocytotic (Rab3a, Rab3b, Rab3c, and Rab27b) and endocytic (Rab4b, Rab5a/b, Rab10, Rab11b, and Rab14) Rab machinery of SVs. Analysis of two closely related proteins, Rab3a and Rab27b, revealed colocalization in synaptic nerve terminals, where they reside on distinct but overlapping SV pools. Moreover, whereas Rab3a readily dissociates from SVs during Ca(2+)-triggered exocytosis, and is susceptible to membrane extraction by Rab-GDI, Rab27b persists on SV membranes upon stimulation and is resistant to GDI-coupled Rab retrieval. Finally, we demonstrate that selective modulation of the GTP/GDP switch mechanism of Rab27b impairs SV recycling, suggesting that Rab27b, probably in concert with Rab3s, is involved in SV exocytosis.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Rab27bRatsynaptic vesicle endocytosis involved_inIMP PMID:20926670SynGO 
Rab27bRatsynaptic vesicle endocytosis involved_inIDA PMID:20926670SynGO 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Rab1aRatpresynapse located_inIDA PMID:20926670SynGO-UCL 
Rab2bRatpresynapse located_inIDA PMID:20926670ARUK-UCL 
Rab33bRatpresynapse is_active_inEXP PMID:20926670SynGO 
Rab33bRatpresynapse is_active_inIMP PMID:20926670SynGO 
Rab33bRatpresynapse is_active_inIEP PMID:20926670SynGO 
Rab33bRatpresynapse is_active_inIDA PMID:20926670SynGO 
Rab6aRatpresynapse is_active_inEXP PMID:20926670SynGO 
Rab6aRatpresynapse is_active_inIEP PMID:20926670SynGO 
Rab6aRatpresynapse is_active_inIDA PMID:20926670SynGO 
Rab6bRatpresynapse is_active_inEXP PMID:20926670SynGO 
Rab6bRatpresynapse is_active_inIEP PMID:20926670SynGO 
Rab6bRatpresynapse is_active_inIDA PMID:20926670SynGO 
Rab8bRatpresynapse is_active_inEXP PMID:20926670SynGO 
Rab8bRatpresynapse is_active_inIDA PMID:20926670SynGO 
Rab8bRatpresynapse is_active_inIMP PMID:20926670SynGO 
Gdi1Ratpresynaptic cytosol is_active_inIDA PMID:20926670SynGO 
Rab10Ratsynaptic vesicle membrane is_active_inEXP PMID:20926670SynGO 
Rab10Ratsynaptic vesicle membrane is_active_inIMP PMID:20926670SynGO 
Rab10Ratsynaptic vesicle membrane is_active_inIEP PMID:20926670SynGO 
Rab10Ratsynaptic vesicle membrane is_active_inIDA PMID:20926670SynGO 
Rab11bRatsynaptic vesicle membrane is_active_inEXP PMID:20926670SynGO 
Rab11bRatsynaptic vesicle membrane is_active_inIEP PMID:20926670SynGO 
Rab11bRatsynaptic vesicle membrane is_active_inIDA PMID:20926670SynGO 
Rab14Ratsynaptic vesicle membrane is_active_inEXP PMID:20926670SynGO 
Rab14Ratsynaptic vesicle membrane is_active_inIMP PMID:20926670SynGO 
Rab14Ratsynaptic vesicle membrane is_active_inIEP PMID:20926670SynGO 
Rab14Ratsynaptic vesicle membrane is_active_inIDA PMID:20926670SynGO 
Rab27bRatsynaptic vesicle membrane is_active_inEXP PMID:20926670SynGO 
Rab27bRatsynaptic vesicle membrane located_inIDA PMID:20926670SynGO-UCL 
Rab27bRatsynaptic vesicle membrane is_active_inIEP PMID:20926670SynGO 
Rab27bRatsynaptic vesicle membrane is_active_inIDA PMID:20926670SynGO 
Rab2aRatsynaptic vesicle membrane is_active_inEXP PMID:20926670SynGO 
Rab2aRatsynaptic vesicle membrane is_active_inIEP PMID:20926670SynGO 
Rab2aRatsynaptic vesicle membrane is_active_inIDA PMID:20926670SynGO 
Rab35Ratsynaptic vesicle membrane is_active_inEXP PMID:20926670SynGO 
Rab35Ratsynaptic vesicle membrane located_inIDA PMID:20926670SynGO-UCL 
Rab35Ratsynaptic vesicle membrane is_active_inIEP PMID:20926670SynGO 
Rab35Ratsynaptic vesicle membrane is_active_inIDA PMID:20926670SynGO 
Rab3aRatsynaptic vesicle membrane located_inIDA PMID:20926670SynGO-UCL 
Rab3bRatsynaptic vesicle membrane located_inIDA PMID:20926670SynGO-UCL 
Rab4aRatsynaptic vesicle membrane is_active_inIDA PMID:20926670SynGO 
Rab4aRatsynaptic vesicle membrane is_active_inIEP PMID:20926670SynGO 
Rab4bRatsynaptic vesicle membrane is_active_inEXP PMID:20926670SynGO 
Rab4bRatsynaptic vesicle membrane is_active_inIMP PMID:20926670SynGO 
Rab4bRatsynaptic vesicle membrane is_active_inIEP PMID:20926670SynGO 
Rab4bRatsynaptic vesicle membrane is_active_inIDA PMID:20926670SynGO 
Rab5aRatsynaptic vesicle membrane is_active_inEXP PMID:20926670SynGO 
Rab5aRatsynaptic vesicle membrane located_inIDA PMID:20926670SynGO-UCL 
Rab5aRatsynaptic vesicle membrane is_active_inIMP PMID:20926670SynGO 
Rab5aRatsynaptic vesicle membrane is_active_inIEP PMID:20926670SynGO 
Rab5aRatsynaptic vesicle membrane is_active_inIDA PMID:20926670SynGO 
Rab5al1Ratsynaptic vesicle membrane is_active_inEXP PMID:20926670SynGO 
Rab5al1Ratsynaptic vesicle membrane located_inIDA PMID:20926670SynGO-UCL 
Rab5al1Ratsynaptic vesicle membrane is_active_inIMP PMID:20926670SynGO 
Rab5al1Ratsynaptic vesicle membrane is_active_inIEP PMID:20926670SynGO 
Rab5al1Ratsynaptic vesicle membrane is_active_inIDA PMID:20926670SynGO 
Rab5bRatsynaptic vesicle membrane is_active_inEXP PMID:20926670SynGO 
Rab5bRatsynaptic vesicle membrane located_inIDA PMID:20926670SynGO-UCL 
Rab5bRatsynaptic vesicle membrane is_active_inIDA PMID:20926670SynGO 
Rab5cRatsynaptic vesicle membrane is_active_inEXP PMID:20926670SynGO 
Rab5cRatsynaptic vesicle membrane located_inIDA PMID:20926670SynGO-UCL 
Rab5cRatsynaptic vesicle membrane is_active_inIDA PMID:20926670SynGO 
Rab7aRatsynaptic vesicle membrane is_active_inEXP PMID:20926670SynGO 
Rab7aRatsynaptic vesicle membrane is_active_inIDA PMID:20926670SynGO 
Rab7aRatsynaptic vesicle membrane is_active_inIEP PMID:20926670SynGO 
Rab7aRatsynaptic vesicle membrane is_active_inIMP PMID:20926670SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Gdi1  (GDP dissociation inhibitor 1)
Rab10  (RAB10, member RAS oncogene family)
Rab11b  (RAB11B, member RAS oncogene family)
Rab14  (RAB14, member RAS oncogene family)
Rab1a  (RAB1A, member RAS oncogene family)
Rab27b  (RAB27B, member RAS oncogene family)
Rab2a  (RAB2A, member RAS oncogene family)
Rab2b  (RAB2B, member RAS oncogene family)
Rab33b  (RAB33B, member RAS oncogene family)
Rab35  (RAB35, member RAS oncogene family)
Rab3a  (RAB3A, member RAS oncogene family)
Rab3b  (RAB3B, member RAS oncogene family)
Rab4a  (RAB4A, member RAS oncogene family)
Rab4b  (RAB4B, member RAS oncogene family)
Rab5a  (RAB5A, member RAS oncogene family)
Rab5al1  (Rab5a, member RAS oncogene family-like 1)
Rab5b  (RAB5B, member RAS oncogene family)
Rab5c  (RAB5C, member RAS oncogene family)
Rab6a  (RAB6A, member RAS oncogene family)
Rab6b  (RAB6B, member RAS oncogene family)
Rab7a  (RAB7A, member RAS oncogene family)
Rab8b  (RAB8B, member RAS oncogene family)


Additional Information