RGD Reference Report - Crystal structure of a heterotetrameric NMDA receptor ion channel. - Rat Genome Database

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Crystal structure of a heterotetrameric NMDA receptor ion channel.

Authors: Karakas, Erkan  Furukawa, Hiro 
Citation: Karakas E and Furukawa H, Science. 2014 May 30;344(6187):992-7. doi: 10.1126/science.1251915.
RGD ID: 13432263
Pubmed: PMID:24876489   (View Abstract at PubMed)
PMCID: PMC4113085   (View Article at PubMed Central)
DOI: DOI:10.1126/science.1251915   (Journal Full-text)

N-Methyl-D-aspartate (NMDA) receptors belong to the family of ionotropic glutamate receptors, which mediate most excitatory synaptic transmission in mammalian brains. Calcium permeation triggered by activation of NMDA receptors is the pivotal event for initiation of neuronal plasticity. Here, we show the crystal structure of the intact heterotetrameric GluN1-GluN2B NMDA receptor ion channel at 4 angstroms. The NMDA receptors are arranged as a dimer of GluN1-GluN2B heterodimers with the twofold symmetry axis running through the entire molecule composed of an amino terminal domain (ATD), a ligand-binding domain (LBD), and a transmembrane domain (TMD). The ATD and LBD are much more highly packed in the NMDA receptors than non-NMDA receptors, which may explain why ATD regulates ion channel activity in NMDA receptors but not in non-NMDA receptors.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Grin1Ratprotein heterotetramerization involved_inIDA PMID:24876489UniProt 
Grin2bRatprotein heterotetramerization involved_inIDA PMID:24876489UniProt 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Grin1RatNMDA selective glutamate receptor complex part_ofIDA PMID:24876489UniProt 
Grin1RatNMDA selective glutamate receptor complex part_ofIPI PMID:24876489ComplexPortal 
Grin2bRatNMDA selective glutamate receptor complex part_ofIDA PMID:24876489UniProt 
Grin2bRatNMDA selective glutamate receptor complex part_ofIPI PMID:24876489ComplexPortal 
Grin1Ratplasma membrane located_inIDA PMID:24876489UniProt 
Grin1Ratplasma membrane part_ofIDA PMID:24876489ComplexPortal 
Grin2bRatplasma membrane located_inIDA PMID:24876489UniProt 
Grin2bRatplasma membrane part_ofIDA PMID:24876489ComplexPortal 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Grin2bRatglutamate binding enablesIDA PMID:24876489UniProt 
Grin1Ratglycine binding enablesIDA PMID:24876489UniProt 
Grin1RatNMDA glutamate receptor activity enablesIDA PMID:24876489UniProt 
Grin2bRatNMDA glutamate receptor activity enablesIDA PMID:24876489UniProt 
Grin1Ratprotein binding enablesIPIUniProtKB:Q00960PMID:24876489IntAct 
Grin2bRatprotein binding enablesIPIUniProtKB:P35439PMID:24876489IntAct 

Objects Annotated

Genes (Rattus norvegicus)
Grin1  (glutamate ionotropic receptor NMDA type subunit 1)
Grin2b  (glutamate ionotropic receptor NMDA type subunit 2B)


Additional Information