RGD Reference Report - Effects of all-trans retinoic acid as a potential chemopreventive agent on the formation of azoxymethane-induced aberrant crypt foci: differential expression of c-myc and c-fos MRNA and protein. - Rat Genome Database

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Effects of all-trans retinoic acid as a potential chemopreventive agent on the formation of azoxymethane-induced aberrant crypt foci: differential expression of c-myc and c-fos MRNA and protein.

Authors: Stopera, S A  Bird, R P 
Citation: Stopera SA and Bird RP, Int J Cancer. 1993 Mar 12;53(5):798-803.
RGD ID: 13432056
Pubmed: PMID:8449605   (View Abstract at PubMed)

The main objectives were to determine the modulating effects of all-trans retinoic acid on the number, size and multiplicity of aberrant crypt foci as well as the in vivo expression of the genes c-myc and c-fos. These foci, which are hypothesized to be the pre-malignant lesions of colon cancer, were induced in Sprague-Dawley rats with a single injection of azoxymethane. Rats were fed either a control diet (AIN-76) or the control diet to which had been added 75 mg/kg or 150 mg/kg all-trans retinoic acid. Within 4 weeks, we observed that the diets containing all-trans retinoic acid reduced the total number and multiplicity of aberrant crypt foci in the colon. However, all-trans retinoic acid increased the size of the lesions that persisted, possibly due to a greater proportion of lesions with dilated crypts. In situ hybridization and immunohistochemistry were performed on the colons for the in vivo analysis of gene expression in these lesions. The expression of myc-specific mRNA and protein in aberrant crypt foci significantly decreased with both levels of all-trans retinoic acid. In contrast, fos-specific mRNA and protein in aberrant crypt foci significantly increased when 150 mg/kg all-trans retinoic acid was added to the diet. The most important findings of this investigation are that intervention with all-trans retinoic acid in the pre-malignant stage of colon carcinogenesis is effective in decreasing the number and growth of aberrant crypt foci and altering the expression of the c-myc and c-fos genes.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
FOSHumanAberrant Crypt Foci treatmentISORGD:2626 RGD 
FosRatAberrant Crypt Foci treatmentIDA  RGD 
FosMouseAberrant Crypt Foci treatmentISORGD:2626 RGD 
MYCHumanAberrant Crypt Foci treatmentISORGD:3130 RGD 
MycRatAberrant Crypt Foci treatmentIDA  RGD 
MycMouseAberrant Crypt Foci treatmentISORGD:3130 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Fos  (Fos proto-oncogene, AP-1 transcription factor subunit)
Myc  (MYC proto-oncogene, bHLH transcription factor)

Genes (Mus musculus)
Fos  (FBJ osteosarcoma oncogene)
Myc  (myelocytomatosis oncogene)

Genes (Homo sapiens)
FOS  (Fos proto-oncogene, AP-1 transcription factor subunit)
MYC  (MYC proto-oncogene, bHLH transcription factor)


Additional Information