RGD Reference Report - Molecular cloning and expression of mouse GD1alpha/GT1aalpha/GQ1balpha synthase (ST6GalNAc VI) gene.

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Molecular cloning and expression of mouse GD1alpha/GT1aalpha/GQ1balpha synthase (ST6GalNAc VI) gene.
Authors:	Okajima, T Chen, HH Ito, H Kiso, M Tai, T Furukawa, K Urano, T Furukawa, K
Citation:	Okajima T, etal., J Biol Chem 2000 Mar 10;275(10):6717-23.
RGD ID:	1331364
Pubmed:	PMID:10702226 (View Abstract at PubMed)
A novel member of the mouse CMP-NeuAc:beta-N-acetylgalactosaminide alpha2,6-sialyltransferase (ST6GalNAc) subfamily, designated ST6GalNAc VI, was identified by BLAST analysis of expressed sequence tags. The sequence of the cDNA clone of ST6GalNAc VI encoded a type II membrane protein with 43 amino acids composing the cytoplasmic domain, 21 amino acids composing the transmembrane region, and 269 amino acids composing the catalytic domain. The predicted amino acid sequence showed homology to the previously cloned ST6GalNAc III, IV, and V, with common amino acid sequences in sialyl motif L and S among these four enzymes. A fusion protein with protein A and extracts from L cells transfected with ST6GalNAc VI in an expression vector showed enzyme activity of alpha2,6-sialyltransferase for GM1b, GT1b, and GD1a but not toward glycoproteins. Thin layer chromatography-immunostaining revealed that the products were GD1alpha, GQ1balpha, and GT1aalpha. Northern blotting revealed that this gene was expressed in a wide range of mouse tissues such as colon, liver, heart, spleen, and brain. It is concluded that this enzyme is a novel sialyltransferase involved in the synthesis of alpha-series gangliosides in the nervous tissues and many other tissues.

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Biological Process

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Object Symbol	Species	Term	Qualifier	Evidence	With	Notes	Source	Original Reference(s)
Gpaa1	Rat	GPI anchor biosynthetic process		TAS			RGD

Cellular Component

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Object Symbol	Species	Term	Qualifier	Evidence	With	Notes	Source	Original Reference(s)
Gpaa1	Rat	GPI-anchor transamidase complex		TAS			RGD

Molecular Function

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Object Symbol	Species	Term	Qualifier	Evidence	With	Notes	Source	Original Reference(s)
Gpaa1	Rat	GPI-anchor transamidase activity		TAS			RGD

Objects Annotated

Genes (Rattus norvegicus)

Gpaa1 (glycosylphosphatidylinositol anchor attachment 1)

Objects referenced in this article

Gene	St6galnac6	ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 6	Rattus norvegicus

Additional Information

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Molecular cloning and expression of mouse GD1alpha/GT1aalpha/GQ1balpha synthase (ST6GalNAc VI) gene.