RGD Reference Report - Regulation of glutamate receptor internalization by the spine cytoskeleton is mediated by its PKA-dependent association with CPG2. - Rat Genome Database

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Regulation of glutamate receptor internalization by the spine cytoskeleton is mediated by its PKA-dependent association with CPG2.

Authors: Loebrich, Sven  Djukic, Biljana  Tong, Zachary J  Cottrell, Jeffrey R  Turrigiano, Gina G  Nedivi, Elly 
Citation: Loebrich S, etal., Proc Natl Acad Sci U S A. 2013 Nov 19;110(47):E4548-56. doi: 10.1073/pnas.1318860110. Epub 2013 Nov 4.
RGD ID: 13209020
Pubmed: PMID:24191017   (View Abstract at PubMed)
PMCID: PMC3839735   (View Article at PubMed Central)
DOI: DOI:10.1073/pnas.1318860110   (Journal Full-text)

A key neuronal mechanism for adjusting excitatory synaptic strength is clathrin-mediated endocytosis of postsynaptic glutamate receptors (GluRs). The actin cytoskeleton is critical for clathrin-mediated endocytosis, yet we lack a mechanistic understanding of its interaction with the endocytic process and how it may be regulated. Here we show that F-actin in dendritic spines physically binds the synaptic nuclear envelope 1 gene product candidate plasticity gene 2 (CPG2) in a PKA-dependent manner, and that this association is required for synaptic GluR internalization. Mutating two PKA sites on CPG2 disrupts its cytoskeletal association, attenuating GluR endocytosis and affecting the efficacy of synaptic transmission in vivo. These results identify CPG2 as an F-actin binding partner that functionally mediates interaction of the spine cytoskeleton with postsynaptic endocytosis. Further, the regulation of CPG2/F-actin association by PKA provides a gateway for cellular control of synaptic receptor internalization through second messenger signaling pathways. Recent identification of human synaptic nuclear envelope 1 as a risk locus for bipolar disorder suggests that CPG2 could play a role in synaptic dysfunction underlying neuropsychiatric disease.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Syne1Ratnegative regulation of mini excitatory postsynaptic potential  IMP  RGD 
Syne1Ratregulation of postsynaptic neurotransmitter receptor internalization  IMP  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Syne1Ratdendritic spine head  IDA  RGD 
Syne1Ratglutamatergic synapse is_active_inIDA PMID:24191017SynGO 
Syne1Ratglutamatergic synapse is_active_inIEP PMID:24191017SynGO 
Syne1Ratglutamatergic synapse is_active_inIMP PMID:24191017SynGO 
Syne1Ratpostsynaptic actin cytoskeleton is_active_inIDA PMID:24191017SynGO 
Syne1Ratpostsynaptic actin cytoskeleton is_active_inIEP PMID:24191017SynGO 
Syne1Ratpostsynaptic actin cytoskeleton is_active_inIMP PMID:24191017SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Syne1  (spectrin repeat containing nuclear envelope protein 1)


Additional Information