RGD Reference Report - Recruitment of dynein to late endosomes and lysosomes through light intermediate chains. - Rat Genome Database

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Recruitment of dynein to late endosomes and lysosomes through light intermediate chains.

Authors: Tan, Serena C  Scherer, Julian  Vallee, Richard B 
Citation: Tan SC, etal., Mol Biol Cell. 2011 Feb 15;22(4):467-77. doi: 10.1091/mbc.E10-02-0129. Epub 2010 Dec 17.
RGD ID: 13207422
Pubmed: PMID:21169557   (View Abstract at PubMed)
PMCID: PMC3038645   (View Article at PubMed Central)
DOI: DOI:10.1091/mbc.E10-02-0129   (Journal Full-text)

Cytoplasmic dynein is involved in a wide range of cellular processes, but how it is regulated and how it recognizes an extremely wide range of cargo are incompletely understood. The dynein light intermediate chains, LIC1 and LIC2 (DYNC1LI1 and DYNC1LI2, respectively), have been implicated in cargo binding, but their full range of functions is unknown. Using LIC isoform-specific antibodies, we report the first characterization of their subcellular distribution and identify a specific association with elements of the late endocytic pathway, but not other vesicular compartments. LIC1 and LIC2 RNA interference (RNAi) each specifically disrupts the distribution of lysosomes and late endosomes. Stimulation of dynein-mediated late-endosomal transport by the Rab7-interacting lysosomal protein (RILP) is reversed by LIC1 RNAi, which displaces dynein, but not dynactin, from these structures. Conversely, expression of ¿N-RILP or the dynactin subunit dynamitin each fails to displace dynein, but not dynactin. Thus, using a variety of complementary approaches, our results indicate a novel specific role for the LICs in dynein recruitment to components of the late endocytic pathway.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Dync1li1Ratcentrosome  IDA  RGD 
Dync1li2Ratcentrosome  IDA  RGD 
Dync1li1Ratkinetochore  IDA  RGD 
Dync1li2Ratkinetochore  IDA  RGD 
Dync1li1Ratlate endosome located_inIDA  RGD 
Dync1li2Ratlate endosome located_inIDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Dync1li1  (dynein cytoplasmic 1 light intermediate chain 1)
Dync1li2  (dynein, cytoplasmic 1 light intermediate chain 2)

Objects referenced in this article
Gene DYNC1LI1 dynein cytoplasmic 1 light intermediate chain 1 Homo sapiens
Gene DYNC1LI2 dynein cytoplasmic 1 light intermediate chain 2 Homo sapiens
Gene Dync1li1 dynein cytoplasmic 1 light intermediate chain 1 Mus musculus
Gene Dync1li2 dynein, cytoplasmic 1 light intermediate chain 2 Mus musculus

Additional Information