RGD Reference Report - Activation of retinoic acid receptor gamma induces proliferation of immortalized hippocampal progenitor cells. - Rat Genome Database

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Activation of retinoic acid receptor gamma induces proliferation of immortalized hippocampal progenitor cells.

Authors: Chung, JJ  Cho, S  Kwon, YK  Kim, DH  Kim, K 
Citation: Chung JJ, etal., Brain Res Mol Brain Res 2000 Nov 10;83(1-2):52-62.
RGD ID: 1300463
Pubmed: PMID:11072095   (View Abstract at PubMed)

In the present study, we report evidence that activation of RARgamma promotes cell proliferation in immortalized hippocampal progenitor cell line HiB5. We found that treatment of HiB5 cells with all-trans- (all-trans-RA) or 9-cis-retinoic acid (9-cis-RA) significantly increased the number of dead floating cells as well as viable cells in serum-free defined medium (N2). Flow cytometric analysis of DNA contents revealed that the proportion of apoptotic cells over the whole cell population was not affected by both retinoids. Instead, the proportion of S phase cells was significantly increased by retinoids. Under this condition, bcl-2 mRNA levels were significantly increased over time by retinoid treatment, whereas bax mRNA levels were not affected. This suggests that retinoids increase viable cells by enhancing proliferation rather than by suppressing apoptosis. In an attempt to dissect the molecular mechanism underlying retinoid-induced HiB5 cell proliferation, we examined the expression patterns of retinoid receptors following retinoid treatment. Retinoids induced RARgamma mRNA, which paralleled the increase in the transactivation of strong retinoic acid response element (RARE) reporter construct. Accordingly, treatment of HiB5 cells with RARgamma-selective agonist (CD666) increased HiB5 cell number in a dose-dependent manner, which was blocked by co-treatment with RARgamma-selective antagonist (CD2665). Taken together, these data clearly indicate that activation of RARgamma increases proliferation of immortalized hippocampal progenitor cells.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
RargRatcellular response to retinoic acid  IEP  RGD 
RargRatpositive regulation of cell population proliferation  IMP  RGD 
RargRatretinoic acid receptor signaling pathway  IMP  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
RargRatnuclear receptor activity  IMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Rarg  (retinoic acid receptor, gamma)


Additional Information