RGD Reference Report - The lectin-like receptor KLRE1 inhibits natural killer cell cytotoxicity. - Rat Genome Database

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The lectin-like receptor KLRE1 inhibits natural killer cell cytotoxicity.

Authors: Westgaard, IH  Dissen, E  Torgersen, KM  Lazetic, S  Lanier, LL  Phillips, JH  Fossum, S 
Citation: Westgaard IH, etal., J Exp Med 2003 Jun 2;197(11):1551-61.
RGD ID: 1299590
Pubmed: PMID:12782717   (View Abstract at PubMed)
PMCID: PMC2193914   (View Article at PubMed Central)
DOI: DOI:10.1084/jem.20021253   (Journal Full-text)

We report the cloning and functional characterization in the mouse and the rat of a novel natural killer (NK) cell receptor termed KLRE1. The receptor is a type II transmembrane protein with a COOH-terminal lectin-like domain, and constitutes a novel KLR family. Rat Klre1 was mapped to the NK gene complex. By Northern blot and flow cytometry using newly generated monoclonal antibodies, KLRE1 was shown to be expressed by NK cells and a subpopulation of CD3+ cells, with pronounced interstrain variation. Western blot analysis indicated that KLRE1 can be expressed on the NK cell surface as a disulphide-linked dimer. The predicted proteins do not contain immunoreceptor tyrosine-based inhibitory motifs (ITIMs) or a positively charged amino acid in the transmembrane domain. However, in a redirected lysis assay, the presence of whole IgG, but not of F(ab')2 fragments of a monoclonal anti-KLRE1 antibody inhibited lysis of Fc-receptor bearing tumor target cells. Moreover, the tyrosine phosphatase SHP-1 was coimmunoprecipitated with KLRE1 from pervanadate-treated interleukin 2-activated NK cells. Together, our results indicate that KLRE1 may form a functional heterodimer with an as yet unidentified ITIM-bearing partner that recruits SHP-1 to generate an inhibitory receptor complex.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Klre1Ratnegative regulation of natural killer cell mediated cytotoxicity directed against tumor cell target  IDA  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Klre1Ratcell surface  IDA  RGD 

Molecular Function

  

Objects Annotated

Genes (Rattus norvegicus)
Klre1  (killer cell lectin like receptor E1)
Ptpn6  (protein tyrosine phosphatase, non-receptor type 6)

Objects referenced in this article
Gene Klre1 killer cell lectin-like receptor family E member 1 Mus musculus

Additional Information