RGD Reference Report - Screening for genes up-regulated in 5/6 nephrectomized mouse kidney. - Rat Genome Database

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Screening for genes up-regulated in 5/6 nephrectomized mouse kidney.

Authors: Zhang, H  Wada, J  Kanwar, YS  Tsuchiyama, Y  Hiragushi, K  Hida, K  Shikata, K  Makino, H 
Citation: Zhang H, etal., Kidney Int 1999 Aug;56(2):549-58.
RGD ID: 1299473
Pubmed: PMID:10432394   (View Abstract at PubMed)
DOI: DOI:10.1046/j.1523-1755.1999.00561.x   (Journal Full-text)

BACKGROUND: In diabetic and nondiabetic renal diseases, glomerular hyperfiltration is believed to play a central role in the subsequent progression of glomerulosclerosis and interstitial renal scarring. To identify genes involved in the process of hyperfiltration and hypertrophy, a polymerase chain reaction (PCR)-based subtraction method, that is, representational difference analysis of cDNA (cDNA-RDA), was employed. METHODS: Ten-week-old ICR mice were 5/6 nephrectomized and sham operated. After two weeks, mRNAs were isolated from control and remnant kidneys and were subjected to the cDNA-RDA procedure. RESULTS: We identified 10 known and 9 novel genes. Among 19 clones, 12 clones (8 known and 4 novel) showed 1.5- to 6-fold up-regulation by Northern blot analyses. The remaining seven clones were rarely expressed genes and were barely detected by Northern blot analyses, and their up-regulated expression was confirmed by Southern blot analysis using the PCR-amplified representative amplicons. The known genes included kidney androgen-regulated protein, major urinary protein, lysozyme M, metalloproteinase-3 tissue inhibitor, chaperonin 10, cytochrome oxidase I, epsilon-sarcoglycan, ribosomal protein S3a, G-proteingamma10 subunit, and splicing factor 9G8. All of the isolated known genes have not been reported to be up-regulated in the nephrectomized mouse kidney and suggest the possible role of androgen action, mitochondrial functions, matrix metabolism, cell-matrix interactions, and intracellular signaling events in the initiation of the progressive renal injury of the remnant kidney. Furthermore, cDNA-RDA facilitates the discovery of novel genes, including two kidney-specific genes. CONCLUSIONS: The isolated known and novel genes may be involved in the pathobiological process of initial hyperfiltration and hypertrophy of remnant kidney.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
SRSF7HumanGlomerular Hyperfiltration  ISOSrsf7 (Mus musculus)mRNA:increased expression:kidney (mouse)RGD 
Srsf7RatGlomerular Hyperfiltration  ISOSrsf7 (Mus musculus)mRNA:increased expression:kidney (mouse)RGD 
Srsf7MouseGlomerular Hyperfiltration  IEP mRNA:increased expression:kidney (mouse)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Srsf7  (serine and arginine rich splicing factor 7)

Genes (Mus musculus)
Srsf7  (serine and arginine-rich splicing factor 7)

Genes (Homo sapiens)
SRSF7  (serine and arginine rich splicing factor 7)

Objects referenced in this article
Gene Cltrn collectrin, amino acid transport regulator Rattus norvegicus

Additional Information